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Recombinant Mouse Fetuin A/AHSG Protein, CF

Catalog # 1563-PI | R&D Systems, Inc. a Bio-Techne Brand
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1563-PI-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Fetuin A/AHSG protein
Ala19-Ile345, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala19

Predicted Molecular Mass

37 kDa

SDS-PAGE

58 kDa, reducing conditions

Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <0.20 µM, as measured under the described conditions.

Formulation, Preparation and Storage

1563-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 500 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fetuin A/AHSG

Mouse Fetuin A, also known as alpha2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It has been also called "countertrypsin" because of its ability to inhibit trypsin (1). It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (2, 3). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (4). The major form of plasma Fetuin A corresponds to two disulfide bond-linked chains derived from the single chain (5). Fetuin-A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300-600 μg/mL), which fall significantly (30-50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (7, 8).

References

  1. Yamamoto, K. and H. Sinohara (1993) J. Biol. Chem. 268:17750.
  2. Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
  3. Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
  4. Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
  5. Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta 671:78.
  6. Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
  7. Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
  8. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.

Long Name

alpha-2-HS Glycoprotein

Alternate Names

AHSG, alpha-2-HS-glycoprotein

Entrez Gene IDs

197 (Human); 11625 (Mouse); 25373 (Rat)

Gene Symbol

AHSG

UniProt

Additional Fetuin A/AHSG Products

Product Documents for Recombinant Mouse Fetuin A/AHSG Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Fetuin A/AHSG Protein, CF

For research use only

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