Product Specifications for Recombinant Mouse FAM3C Protein, CF
Mouse myeloma cell line, NS0-derived mouse FAM3C protein Gln25-Asp227, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
No results obtained: Gln25 predicted, sequencing might be blocked
Predicted Molecular Mass
23-25 kDa, reducing conditions
Measured by the ability of the immobilized protein to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells. The ED50 for this effect is 1-5 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 250 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Family with sequence similarity 3, member C (FAM3C), also known as Interleukin-Like EMT Inducer (ILEI), is an approximately 25 kDa secreted protein in the FAM group of molecules (1, 2). Members of this family share a four helix bundle secondary structure similar to that found in many cytokines. Mature mouse FAM3C shares 94% and 97% amino acid sequence identity with human and rat FAM3C, respectively. It is widely expressed, notably in the nonsensory epithelium of auditory semicircular canals (1) and in the photoreceptor layer of the retina (3). FAM3C is up‑regulated and shows altered intracellular localization in various tumors (4, 5). Its translation is normally silenced by the binding of hnRNP E1 to the FAM3C mRNA, but this inhibition is removed following cellular exposure to TGF-beta (6, 7). FAM3C, like TGF-beta, promotes epithelial-mesenchymal transition (EMT), tumor growth, and metastasis (4-6). FAM3C cooperates with oncogenic Ras to promote the up‑regulation of PDGF-C and PDGF R beta, leading to increased PDGF receptor signaling (5). R&D Systems in-house testing indicates that FAM3C supports the adhesion of Caki cells.
Pilipenko, V.V. et al. (2004) Gene 335:159.
Zhu, Y. et al. (2002) Genomics 80:144.
Katahira, T. et al. (2010) Biochem. Biophys. Res. Commun. 392:301.
Waerner, T. et al. (2006) Cancer Cell 10:227.
Lahsnig, C. et al. (2009) Oncogene 28:638.
Chaudhury, A. et al. (2010) Nat. Cell Biol. 12:286.
Hussey, G.S. et al. (2011) Mol. Cell 41:419.
Family With Sequence Similarity 3, Member C
Entrez Gene IDs
10447 (Human); 27999 (Mouse); 312159 (Rat)
D6WSU176e, FAM3C, GS3876, ILEI, ILEIInterleukin-like EMT inducer, family with sequence similarity 3, member C, predicted osteoblast protein
Citations for Recombinant Mouse FAM3C Protein, CF
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Product Documents for Recombinant Mouse FAM3C Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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