Recombinant Mouse Epiregulin Protein

Name: Recombinant Mouse Epiregulin Protein
Brand: R&D Systems
SKU: 1068-EP

Catalog # 1068-EP

Citations (5)

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Key Product Details

Source:	E. coli
Accession #:	NP_031976
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Epiregulin Protein

Product Specifications

Source	E. coli-derived mouse Epiregulin protein Val56-Leu101, with an N-terminal Met
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	5.5 kDa
Activity	Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED₅₀ for this effect is 0.3-1 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1068-EP

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1068-EP/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Epiregulin

Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. They are all synthesized as transmembrane precursors and converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The mouse Epiregulin cDNA encodes for a transmembrane precursor of 162 amino acid in length, with the mature soluble form comprising residues 56 - 101 (2). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB-family receptors (ErbB1 through B4) (3). Although it stimulates phosphorylation of all four receptors, it appears to interact primarily with ErbB1 and ErbB4. Epiregulin has the broadest specificity of the EGF-like ligands but seems to preferentially activate heterodimeric receptor complexes (4). Epiregulin exhibits a variety of biological effects. It was originally shown to both inhibit growth of several epithelial tumor cells and stimulate growth of fibroblasts and other types of cells (1). Epiregulin expression is upregulated in a number of carcinoma cell lines. It has also been shown to be an autocrine growth factor in human epidermal keratinocytes (5). Epiregulin has also been shown to play a role in the early steps of pregnancy, regulating attachment of the blastocyst to the uterine epithelium during the implantation process (6).

Item	Value
References	Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495. Toyoda, H. et al. (1995) FEBS Lett. 377:403. Komurasaki, T. et al. (1997) Oncogene 15:2841. Shelly, M. et al. (1998) J. Biol. Chem. 273:10496. Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748. Das, S.K. et al. (1997) Dev. Biol. 190:178.
Entrez Gene IDs	2069 (Human); 13874 (Mouse)
Alternate Names	ER, EREG, Epiregulin, proepiregulin

Citations for Recombinant Mouse Epiregulin Protein (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Epiregulin Protein include:

Species: Transgenic Mouse
Sample Types: Organoid
Applications: Bioassay

F Westendorp et al. (2021), Epithelium-derived Indian Hedgehog restricts stromal expression of ErbB family members that drive colonic tumor cell proliferation Oncogene, 0(0).
PMID: 33479497
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

LJ Martin et al. (2017), Epiregulin and EGFR interactions are involved in pain processing J. Clin. Invest., 0(0).
PMID: 28783046
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Shuhaibar L et al. (2016), Dephosphorylation of juxtamembrane serines and threonines of the NPR2 guanylyl cyclase is required for rapid resumption of oocyte meiosis in response to luteinizing hormone. Dev Biol, 409(1):194-201.
PMID: 26522847
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Harada M et al. (2015), Temporal expression of growth factors triggered by epiregulin regulates inflammation development. J Immunol, 194(3):1039-46.
PMID: 25556244
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Patel RD et al. (2006), The aryl hydrocarbon receptor directly regulates expression of the potent mitogen epiregulin. Toxicol. Sci., 89(1):75-82.
PMID: 16192470

Recombinant Mouse Epiregulin Protein

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Product Specifications

Formulation, Preparation and Storage

Carrier Free

1068-EP

Carrier

1068-EP/CF

Carrier Free

Reconstitution Calculator

Background: Epiregulin

Citations for Recombinant Mouse Epiregulin Protein (5)

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