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Recombinant Mouse EphA5 Protein, CF

Catalog # 3037-A5 | R&D Systems, Inc. a Bio-Techne Brand
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3037-A5-100

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse EphA5 protein
Ser58-Pro412, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser58

Predicted Molecular Mass

40.4 kDa

SDS-PAGE

40-50 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rmEphA5 at 2 µg/mL (100 µL/well) can bind rmEphrin-A2/Fc Chimera with a linear range of 0.16-10 ng/mL.

Formulation, Preparation and Storage

3037-A5
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA5

EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor tyrosine kinase family which binds members of the Ephrin ligand family (1 - 4). The A and B class Eph proteins have a common structural organization. Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. Reverse signaling is propagated through the Ephrin ligand. The mouse EphA5 cDNA encodes an 877 amino acid (aa) precursor that includes a 26 aa signal sequence, a 386 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 444 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5). Taking into account differences in splice variants between species, comparable regions of the mouse EphA5 ECD share greater than 97% aa sequence identity with bovine, human, and rat EphA5, and 27 - 43% aa identity with mouse EphA1, 2, 3, 4, 6, 7, and 8. Multiple EphA5 splice variants exist and differ in their extracellular and intracellular sequences (5). Mouse EphA5 is expressed exclusively in brain with the highest levels in the hippocampus and limbic system (5). During embryonal development, EphA5 is preferentially expressed in neurons migrating away from the ventricular zone (5). The interaction of EphA5 with its ligands is also involved in the topographic projection of hippocampal, olfactory, and retinotectal neurons (6). Interference in this system by the transgenic expression of soluble EphA5 implicates hippocampal post-synaptic areas for native EphA5 function (7).

References

  1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
  2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419.
  3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
  5. Zhou, R. et al. (1994) J. Neurosci. Res. 37:129.
  6. Zhou, R. (1997) Cell Tissue Res. 290:251.
  7. Martinez, A. et al. (2005) Neuroscience 135:451.

Alternate Names

Bsk, Cek7, Ehk1, Hek7, Rek7

Entrez Gene IDs

2044 (Human); 13839 (Mouse); 79208 (Rat)

Gene Symbol

EPHA5

UniProt

Product Documents for Recombinant Mouse EphA5 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse EphA5 Protein, CF

For research use only

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Reconstitution Calculator

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