Product Specifications for Recombinant Mouse Endocan Protein, CF
Mouse myeloma cell line, NS0-derived mouse Endocan/ESM-1 protein Trp20-Arg184, with a C-terminal 10-His tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
21-25 kDa, reducing conditions
Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. Recombinant Mouse Endocan/ESM-1 immobilized at 25 μg/mL, 100 μL/well, will mediate >20% cell adhesion (100 μL/well at 0.5 x 106 cells/mL). Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Endocan (endothelial cell proteoglycan; also known as endothelial‑cell specific molecule‑1 [ESM‑1]), is a 50 kDa, monomeric, secreted, cysteine‑rich proteoglycan identified initially in endothelial cells of the kidney and lung (1). Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region (2). The N‑terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N‑linked glycosylation sites. Based on human Endocan, there are at least two potential O‑linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule (3). The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4‑O sulfated N‑acetyl galactosamine with alpha ‑iduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity (4). Mature mouse Endocan is 96% and 74% aa identical to rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa’s 82 through 131, a range which would not remove the dermatan sulfate attachment site (4). It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium (1, 5, 6). It is upregulated by TNF‑ alpha and VEGF, (1, 7) and is known to bind to LFA‑1 ( alpha L beta 2) on the surface of PBMCs, blocking LFA‑1 interaction with ICAM‑1 (8). Normal circulating levels of Endocan are approximately 1 ng/mL (6).
Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
Lassalle, P. (1999) Genbank Accession #: Q9QYY7.
Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
Aitkenhead, M. et al. (2002) Microvasc. Res. 63:159.
Wellner, M. et al. (2003) Horm. Metab. Res. 35:217
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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