Product Specifications for Recombinant Mouse ELFN1 His-tag Protein, CF
Mouse myeloma cell line, NS0-derived mouse ELFN1 protein Gln26-Tyr418, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
73-84 kDa, under reducing conditions
Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 1.25 μg/mL of protein is able to significantly inhibit neurite outgrowth.
Scientific Data Examples for Recombinant Mouse ELFN1 His-tag Protein, CF
Recombinant Mouse ELFN1 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Mouse ELFN1 His-tag Protein (Catalog # 10644-EF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-84 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1 (ELFN1), also known as protein phosphatase 1 regulatory subunit 28 (PPP1R28), is a member of the large family of leucin-rich repeat (LRR) neuronal adhesion protein (1,2). Mouse ELFN1 consists of an extracellular domain (ECD) containing five LRR domains, one LRR C-terminal (LRRCT) domain and an FN3 domain, a type I transmembrane domain, and a cytoplasmic tail (2). Within the mature ECD, mouse ELFN1 shares 89% and 100% amino acid sequence identity with human and rat ELFN1, respectively. ELFN1 is strongly expressed in globus pallidus and interneurons in cortex and hippocampus in both developing and adult brains and is a selective binding partner of group III glutamate receptors (mGluR) (2, 3). Recent studies showed that ELFN1 physically anchor metabotropic glutamate receptor 6 (mGluR6) and mGluR7 across retinal and hippocampal synapses (3, 4), and can be recruited selectively to all group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) to allosterically modulate these receptors (2). ELFN1 mutant mice display seizures, subtle motor abnormalities, hyperactivity and altered thigmotaxis behavior (4). Given the functions and discrete patterns of many known LRR family proteins it has been proposed that ELFN1 could serve as a neuronal adhesion molecule and play an integral role in synapse formation and differentiation via the coordination of both pre- and postsynaptic machineries, thereby involved in neurite outgrowth, axon guidance, fasciculation, and synapse formation (5).
Stachniak, T. et al. (2019) J. Neurosci. 39:4461.
Dunn, H.A. et al. (2018) Proc. Natl. Acad. Sci. USA 115:5022.
Dolan, J. et al. (2007) BMC Genomics 8:320.
Dolan, J. and Mitchel, K.J. (2013) PLoS One 8:e8049.
Williams, M.E. et al. (2010) Neuron 68:9.
Extracellular Leucine-Rich Repeat And Fibronectin Type III Domain Containing 2
Entrez Gene IDs
392617 (Human); 243312 (Mouse); 288512 (Rat)
ELFN1, PPP1R28, USP17L7
Citations for Recombinant Mouse ELFN1 His-tag Protein, CF
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Product Documents for Recombinant Mouse ELFN1 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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