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Recombinant Mouse EGFLAM Flag-tag Protein, CF

Catalog # 10367-EG | R&D Systems, Inc. a Bio-Techne Brand
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10367-EG-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse EGFLAM protein
Mouse EGFLAM
(Leu25-Lys1017)
Accession # Q4VBE4.1
DYKDDDDK
(Flag-tag)
DYKDDDDK
(Flag-tag)
DYKDDDDK
(Flag-tag)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25

Predicted Molecular Mass

111 kDa

SDS-PAGE

103-198 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Dystroglycan Fc Chimera (Catalog # 10223-DG) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse EGFLAM Flag-tag (Catalog # 10367-EG) binds with an ED50 of 0.075-0.45 μg/mL.

Scientific Data Images for Recombinant Mouse EGFLAM Flag-tag Protein, CF

Recombinant Mouse EGFLAM Flag-tag Protein Binding Activity

Recombinant Mouse EGFLAM Flag-tag Protein Binding Activity

When Recombinant Human Dystroglycan Fc Chimera (Catalog # 10223-DG) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse EGFLAM Flag-tag Protein (Catalog # 10367-EG) binds with an ED50 of 0.075-0.45 µg/mL.
Recombinant Mouse EGFLAM Flag-tag Protein SDS-PAGE

Recombinant Mouse EGFLAM Flag-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse EGFLAM (Catalog # 10367-EG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 103-198 kDa.

Formulation, Preparation and Storage

10367-EG
Formulation
Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: EGFLAM

EGFLAM (EGF-like, fibronectin type-III and laminin G-like domain-containing protein), also known as Pikachurin or Nectican, is a highly conserved extracellular matrix-like protein with a molecular weight around 110 kDa. Murine EGFLAM has 1,017 amino acids (∼110 kDa) with a 24 amino acids signal peptide. It can bind to alpha-dystroglycan and has been found to localize mainly in the synaptic cleft of photoreceptor ribbon synapses (1). At the ribbon synapses, EGFLAM protein is colocalized with both dystrophin and dystroglycan, where it plays critical roles in interactions between the photoreceptor ribbon synapse and bipolar dendrites (1, 2). EGFLAM gene is also expressed in other organs and tissues including brain, endocrine tissues and muscle tissues (3). EGFLAM has been considered as a new biomarker for theca interna and granulosa cells (4), a novel susceptibility locus for aortic aneurysm (3), a hypomethylated biomarkers for ovarian cancer (5), and a prognostic biomarker and therapeutic target for glioblastoma (6). EGFLAM contains 11 intracellular disulfide bonds and one O-glycosylation site that attaches to chondroitin sulfate and heparan sulfate. This protein is composed of two fibronectin type-III domains followed by three EGF-like domain and three Laminin G-like domains. Mouse EGFLAM shares 89% and 95% amino acid identity to human and rat EGFLAM, respectively.

References

  1. Sato, S. et al. (2008) Nat. Neurosci. 11:923.
  2. Han, J. and Townes-Anderson, E. (2012) PloS One 7:e50552.
  3. Yamada, Y. et al. (2017) Int. J. Mol. Med. 39:1091.
  4. Hatzirodos, N. et al. (2015) PLoS One 10:e0119800.
  5. Gu, X.H. et al. (2009) Zhonghua Fu Chan Ke Za Zhi 44:754.
  6. Chen, Juhui, et al. (2019) Cancer Biomarkers 24:343.

Long Name

EGF-Like, Fibronectin Type III and Laminin G Domains

Alternate Names

AGRINL, AGRNL, PIKA, Pikachurin

Entrez Gene IDs

133584 (Human); 268780 (Mouse)

Gene Symbol

EGFLAM

UniProt

Product Documents for Recombinant Mouse EGFLAM Flag-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse EGFLAM Flag-tag Protein, CF

For research use only

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