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Recombinant Mouse EGF Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Recombinant Mouse EGF Protein, CF
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2028-EG-200
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Key Product Details

Source: E. coli
Accession #: NP_034243
Applications: Bioactivity

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Citations for Recombinant Mouse EGF Protein, CF (141)

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Customer Reviews for Recombinant Mouse EGF Protein, CF (7)

4.7 out of 5
7 customer ratings
5 stars
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4 stars
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  • Recombinant Mouse EGF Protein, CF
    Name: Anonymous
    Application: CellProlif
    Reason for Rating: Easy to use!
    Verified Customer | Posted 09/17/2022
    Recombinant Mouse EGF Protein, CF Cell Proliferation 2028-EG
  • Recombinant Mouse EGF Protein, CF
    Name: Anonymous
    Application: CellProlif
    Verified Customer | Posted 08/23/2022
    EpiLc Culture
    Recombinant Mouse EGF Protein, CF Cell Proliferation 2028-EG
  • Recombinant Mouse EGF Protein, CF
    Name: Pablo Hurtado
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/28/2020
  • Recombinant Mouse EGF Protein, CF
    Name: Oonagh Paerewijck
    Application: CellProlif
    Verified Customer | Posted 08/14/2018
    culture of murine intestinal cel line10 ng/ml EGF
    Recombinant Mouse EGF Protein, CF Cell Proliferation 2028-EG
  • Recombinant Mouse EGF Protein, CF
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Reason for Rating: No problems, used to grow spermatogonial stem cells.
    Verified Customer | Posted 05/16/2017
  • Recombinant Mouse EGF Protein, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 04/19/2017
    Growth of B-16 Mouse melanoma cells as spheroids in serum-free medium after treatment with Recombinant Mouse EGF protein (#2028-EG) for 48 hours.
    Recombinant Mouse EGF Protein, CF In vitro bioactivity in cell culture 2028-EG
  • Recombinant Mouse EGF Protein, CF
    Name: Catherine Kurtz
    Verified Customer | Posted 05/16/2016
    Recombinant Mouse EGF Protein, CF 2028-EG

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FAQs for Recombinant Mouse EGF Protein, CF

  • The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

    Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.

  • What is the recommended method for reconstitution of a lyophilized protein or antibody?

    Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand.  Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.   

  • Are R&D Systems recombinant proteins and antibodies sterile?

    Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Product Documents for Recombinant Mouse EGF Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.