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Recombinant Mouse EGF Protein, CF Best Seller

Catalog # 2028-EG | R&D Systems, Inc. a Bio-Techne Brand
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2028-EG-200

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse EGF protein
Asn977-Arg1029, with an N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

6.2 kDa

Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415.
The ED50 for this effect is 20-100 pg/mL.

Formulation, Preparation and Storage

2028-EG
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF

Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of The EGF family are characterized by a shared structural motif, the EGF‑like domain, which contains three intramolecular disulfide bonds that are formed by six similarly spaced, conserved cysteine residues (2). These disulfide bonds are essential for proper protein conformation and receptor binding. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The full length EGF protein is 1207 amino acids (aa) (EGF precursor) containing nine EGF domains and nine LDLR class B repeats. However, the mature protein is much smaller, only 53 aa, and is generated by proteolytic cleavage of the EGF domain proximal to the transmembrane region (3). EGF is well conserved across mammals with mature human EGF 70% identical to mature mouse and rat EGF. Physiologically, EGF is found in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

References

  1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
  2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
  3. Gray, A. et al. (1983) Nature 303:722.
  4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
  7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
  8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.

    Long Name

    Epidermal Growth Factor

    Alternate Names

    HOMG4, URG, Urogastrone

    Entrez Gene IDs

    1950 (Human); 13645 (Mouse); 25313 (Rat)

    Gene Symbol

    EGF

    UniProt

    Product Documents for Recombinant Mouse EGF Protein, CF

    Certificate of Analysis

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    Note: Certificate of Analysis not available for kit components.

    Product Specific Notices for Recombinant Mouse EGF Protein, CF

    For research use only

    Citations for Recombinant Mouse EGF Protein, CF (158)

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    Customer Reviews for Recombinant Mouse EGF Protein, CF (7)

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    • Recombinant Mouse EGF Protein, CF
      Name: Anonymous
      Application: Cell Proliferation
      Verified Customer | Posted 09/17/2022
      Recombinant Mouse EGF Protein, CF 2028-EG
    • Recombinant Mouse EGF Protein, CF
      Name: Anonymous
      Application: Cell Proliferation
      Verified Customer | Posted 08/23/2022
      EpiLc Culture
      Recombinant Mouse EGF Protein, CF 2028-EG
    • Recombinant Mouse EGF Protein, CF
      Name: Pablo Hurtado
      Application: In vitro bioactivity in cell culture
      Verified Customer | Posted 05/28/2020
    • Recombinant Mouse EGF Protein, CF
      Name: Oonagh Paerewijck
      Application: Cell Proliferation
      Verified Customer | Posted 08/14/2018
      culture of murine intestinal cel line 10 ng/ml EGF
      Recombinant Mouse EGF Protein, CF 2028-EG
    • Recombinant Mouse EGF Protein, CF
      Name: Anonymous
      Application: Stem/Immune cell maintenance or differentiation
      Verified Customer | Posted 05/16/2017
    • Recombinant Mouse EGF Protein, CF
      Name: Anonymous
      Application: In vitro bioactivity in cell culture
      Verified Customer | Posted 04/19/2017
      Growth of B-16 Mouse melanoma cells as spheroids in serum-free medium after treatment with Recombinant Mouse EGF protein (#2028-EG) for 48 hours.
      Recombinant Mouse EGF Protein, CF 2028-EG
    • Recombinant Mouse EGF Protein, CF
      Name: Catherine Kurtz
      Verified Customer | Posted 05/16/2016
      Recombinant Mouse EGF Protein, CF 2028-EG

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