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Recombinant Mouse Dkk-2 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 2435-DKB/CF

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2435-DKB-010/CF

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Dkk-2 protein
Ser26-Ile259, with Leu35Pro substitution and a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser26

Predicted Molecular Mass

27 kDa

SDS-PAGE

29-35 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells.
Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells. Recombinant Mouse Dkk-2 (Catalog # 2435-DKB/CF) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 for this effect is 100-600 ng/mL.

Scientific Data Images for Recombinant Mouse Dkk-2 Protein, CF

Recombinant Mouse Dkk-2 Protein Bioactivity

Recombinant Mouse Dkk-2 Protein Bioactivity

Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-2 (Catalog # 2435-DKB/CF) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED50 for this effect is 100-600 ng/mL (orange circles).

Formulation, Preparation and Storage

2435-DKB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dkk-2

Dickkopf related protein 2 (Dkk-2) is a member of the Dickkopf family of secreted Wnt modulators (1-3). Dkk proteins contain a signal peptide and two conserved cysteine-rich domains that are separated by a linker region. The second cysteine-rich domain mediates Dkk-2 binding activities, and its interaction with LRP beta propellers has been mapped (2-4, 7). The 226 aa, ~35 kDa mature mouse Dkk-2 shares  99%, 96%, 96%, 96% and 94% aa identity with rat, human, dog, horse and cow Dkk-2, respectively, and can activate the canonical Wnt signaling pathway in Xenopus embryos (5). Dkk proteins modify Wnt engagement of a receptor complex composed of a Frizzled protein and a low-density lipoprotein receptor-related protein, either LRP5 or LRP6 (3). When LRP6 is over-expressed, direct high-affinity binding of Dkk-2 to LRP can enhance canonical Wnt signaling (6-8). However, when Dkk-2 and LRP6 form a ternary complex with Kremen2, Wnt signaling is inhibited due to internalization of Dkk-2/LRP6/Krm2 complexes (9, 10). Thus, depending on the cellular context, Dkk-2 can either activate or inhibit canonical Wnt signaling (3). In contrast, binding of Dkk-1 or Dkk-4 to LRP is consistently antagonistic (3). Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations. Dkk-2 expression has been studied most in bone and eye, although it is expressed as early as periimplantation in mice (11). Mouse Dkk-1 or Dkk-2 deficiencies have opposite effects on bone homeostasis, despite down-regulating Wnt antagonism in both cases (12, 13). Dkk-2 expression is induced by Wnts in bone, and is thought to enhance bone density by promoting terminal differentiation of osteoblasts and mineral deposition (12). In contrast, Dkk-1 negatively regulates late osteoblast proliferation, which limits bone density (13). Dkk-2-deficient mice are blind, exhibiting faulty differentiation of corneal epithelium and ectopic blood vessels in the periocular mesenchyme (14, 15).

References

  1. Monaghan, A.P. et al. (1999) Mech. Dev. 87:45.
  2. Krupnik, V.E. et al. (1999) Gene 238:301.
  3. Niehrs, C. (2006) Oncogene 25:7469.
  4. Chen, L. et al. (2008) J. Biol. Chem. 283:23364.
  5. Wu, W. et al. (2000) Current Biol. 10:1611.
  6. Mao, B. et al. (2001) Nature 411:321.
  7. Li, L. et al. (2002) J. Biol. Chem. 277:5977.
  8. Brott, B. and S.Y. Sokol (2002) Mol. Cell. Biol. 22:6100.
  9. Mao, B. et al. (2002) Nature 417:664.
  10. Mao, B. and C. Niehrs (2003) Gene 302:179.
  11. Zhang, Y. et al. (2009) J. Reprod. Dev. 55:17.
  12. Li, X. et al. (2005) Nat. Genet. 37:945.
  13. van der Horst, G. et al. (2005) J. Bone Miner. Res. 20:1867.
  14. Mukhopadhyay, M. et al. (2006) Development 133:2149.
  15. Gage, P.J. et al. (2008) Dev. Biol. 317:310.

Long Name

Dickkopf-2

Alternate Names

Dkk2

Entrez Gene IDs

27123 (Human); 56811 (Mouse)

Gene Symbol

DKK2

UniProt

Additional Dkk-2 Products

Product Documents for Recombinant Mouse Dkk-2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Dkk-2 Protein, CF

For research use only

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