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Recombinant Mouse Desert Hedgehog/Dhh (C23II) N-Terminus, CF

Bio-Techne includes R&D Systems | Catalog # 733-DH

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733-DH-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse Desert Hedgehog/Dhh protein
Cys23-Gly198 (Cys23Ile-Ile), with an N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

20.1 kDa

Activity

Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.
The ED50 for this effect is <20 µg/mL.

Formulation, Preparation and Storage

733-DH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desert Hedgehog/Dhh

Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are synthesized as 45 kDa precursors that are cleaved autocatalytically. The 19 kDa N-terminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Binding of this fragment to Patched receptors results in the loss of Patched repression of Smoothened signaling (1 - 4). Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) (5). Within the N-terminal peptide, mouse Dhh shares 97% and 100% amino acid (aa) sequence identity with human and rat Dhh, respectively. It shares 74% aa seqeuence identity with mouse Indian (Ihh) and Sonic hedgehog (Shh) (6, 7). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis (8, 9). It induces steroidogenic factor 1 which is instrumental in promoting Leydig cell differentiation (10, 11). It also promotes the deposition of basal lamina surrounding seminiferous tubules (8). In humans, mutations of Dhh are associated with pure gonadal dysgenesis (12). Dhh is expressed in the female by ovarian granulosa cells and the corpus luteum (13). Its upregulation in human ovarian cancer correlates positively with proliferative index and negatively with prognosis (14). Dhh is also expressed by Schwann cells and is upregulated following nerve injury (15, 16). It induces the expression of Patched and Hip in nerve fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves (15).

References

  1. van den Brink, G.R. (2007) Physiol. Rev. 87:1343.
  2. Riobo, N.A. and D.R. Manning (2007) Biochem. J. 403:369.
  3. Porter, J.A. et al. (1995) Nature 374:363.
  4. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. 95:13630.
  5. Pathi, S. et al. (2001) Mech. Dev. 106:107.
  6. Echelard, Y. et al. (1993) Cell 75:1417.
  7. Chang, D.T. et al. (1994) Development 120:3339.
  8. Pierucci-Alves, F. et al. (2001) Biol. Reprod. 65:1392.
  9. Bitgood, M.J. et al. (1996) Curr. Biol. 6:298.
  10. Yao, H.H.-C. et al. (2002) Genes Dev. 16:1433.
  11. Park, S.Y. et al. (2007) Endocrinology 148:3704.
  12. Canto, P. et al. (2004) J. Clin. Endocrinol. 89:4480.
  13. Russell, M.C. et al. (2007) Biol. Reprod. 77:226.
  14. Chen, X. et al. (2007) Cancer Sci. 98:68.
  15. Parmantier, E. et al. (1999) Neuron 23:713.
  16. Bajestan, S.N. et al. (2006) J. Neurobiol. 66:243.

Alternate Names

DHH

Entrez Gene IDs

50846 (Human); 13363 (Mouse)

Gene Symbol

DHH

UniProt

Additional Desert Hedgehog/Dhh Products

Product Documents for Recombinant Mouse Desert Hedgehog/Dhh (C23II) N-Terminus, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Desert Hedgehog/Dhh (C23II) N-Terminus, CF

For research use only

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