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Recombinant Mouse Dectin-2/CLEC6A Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1525-DC

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1525-DC-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Dectin-2/CLEC6A protein
Phe43-Leu209, with an N-terminal 7-His tag and Ala

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

20.6 kDa

SDS-PAGE

24 kDa, reducing conditions

Activity

Measured by its ability to bind biotinylated alpha-D-Mannose-PAA in a functional ELISA.

Formulation, Preparation and Storage

1525-DC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-2/CLEC6A

Dectin-2 alpha, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2 alpha, CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 alpha consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 alpha isoform is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3 - 6). The CRD of Dectin-2 alpha contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 alpha selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 alpha induces tyrosine phosphorylation of the gamma subunit, activation of NF kappaB, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 alpha is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4+CD25+ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 alpha shares 71% aa sequence identity with human and bovine Dectin-2.

References

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Kanazawa, N. et al. (2004) Immunobiology 209:179.
  3. Fernandes, M.J. et al. (1999) Cancer Res. 59:2709.
  4. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:11957.
  5. Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
  6. Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
  7. McGreal, E.P. et al. (2006) Glycobiology 16:422.
  8. Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
  9. Aragane, Y. et al. (2003) J. Immunol. 171:3801.

Alternate Names

CLEC6A, CLECSF10, Dectin2

Entrez Gene IDs

93978 (Human); 56620 (Mouse)

Gene Symbol

CLEC6A

UniProt

Additional Dectin-2/CLEC6A Products

Product Documents for Recombinant Mouse Dectin-2/CLEC6A Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Dectin-2/CLEC6A Protein, CF

For research use only

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