Product Specifications for Recombinant Mouse Decorin Protein, CF
Mouse myeloma cell line, NS0-derived mouse Decorin protein Gly17-Lys354 & Asp31-Lys354, with a C-terminal 10-His tag
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Gly17 & Asp31
Predicted Molecular Mass
40-45 kDa, reducing conditions
Measured by its ability to modulate collagen fibrillogenesis. Ge, G. et al. (2004) J. Biol. Chem. 279:41626. 5 µg/mL of Recombinant Mouse Decorin can significantly delay the rate of collagen fibrillogenesis.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Decorin, also known as PG40 and PGS2, is a secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10 ‑ 12 tandem leucine-rich repeats (LRR) (1). The mouse Decorin cDNA encodes a 354 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide (2). Mature mouse Decorin contains twelve tandem LRR and shares 80% and 87% aa sequence identity with human and rat Decorin, respectively. Decorin is an N-glycosylated protein that also carries a variably-sized hybrid chondroitin/dermatan sulfate chain at Ser34 (2, 3). Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa (4). Decorin regulates assembly of the extracellular collagen matrix and the bioactivity of the matrix associated growth factors FGF-2, GDF-8/Myostatin, TGF-beta, and WISP-1 (3, 5 ‑ 8). It also binds and activates EGF R, ErbB4, and IGF-I R (9 ‑ 11). In vivo, Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression (12 ‑ 15). Decorin is cleared from the extracellular space by LRP‑mediated endocytosis (16).
Schaefer, L. and R.V. Iozzo (2008) J. Biol. Chem. 283:21305.
Scholzen, T. et al. (1994) J. Biol. Chem. 269:28270.
Zamfir, A. et al. (2003) Glycobiology 13:733.
Roughley, P.J. and R.J. White (1989) Biochem. J. 262:823.
Ferdous, Z. et al. (2007) J. Biol. Chem. 282:35887.
Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
Cabello-Verrugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
Desnoyers, L. et al. (2001) J. Biol. Chem. 276:47599.
Zhu, J.-X. et al. (2005) J. Biol. Chem. 280:32468.
Santra, M. et al. (2000) J. Biol. Chem. 275:35153.
Schonherr, E. et al. (2005) J. Biol. Chem. 280:15767.
Olguin, H.C. et al. (2003) Dev. Biol. 259:209.
Schonherr, E. et al. (2004) J. Vasc. Res. 41:499.
Seidler, D.G. et al. (2006) J. Biol. Chem. 281:26408.
Reed, C.C. et al. (2005) Oncogene 24:1104.
Brandan, E. et al. (2006) J. Biol. Chem. 281:31562.
Entrez Gene IDs
1634 (Human); 13179 (Mouse)
Bone proteoglycan II, CSCD, DCN, DSPG2, Decorin, PG-II, PG-S2, PG40, PGII, PGS2, SLRR1B, SLRR1BPGS2, decorin proteoglycan, dermatan sulphate proteoglycans II, proteoglycan core protein, small leucine-rich protein 1B
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