Source	Mouse myeloma cell line, NS0-derived mouse Decorin protein Gly17-Lys354 & Asp31-Lys354, with a C-terminal 10-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gly17 & Asp31
Predicted Molecular Mass	39 kDa
SDS-PAGE	40-45 kDa, reducing conditions
Activity	Measured by its ability to modulate collagen fibrillogenesis. Ge, G. et al. (2004) J. Biol. Chem. 279:41626. 5 µg/mL of Recombinant Mouse Decorin can significantly delay the rate of collagen fibrillogenesis.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1060-DE

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Decorin

Decorin, also known as PG40 and PGS2, is a secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10 ‑ 12 tandem leucine-rich repeats (LRR) (1). The mouse Decorin cDNA encodes a 354 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide (2). Mature mouse Decorin contains twelve tandem LRR and shares 80% and 87% aa sequence identity with human and rat Decorin, respectively. Decorin is an N-glycosylated protein that also carries a variably-sized hybrid chondroitin/dermatan sulfate chain at Ser34 (2, 3). Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa (4). Decorin regulates assembly of the extracellular collagen matrix and the bioactivity of the matrix associated growth factors FGF-2, GDF-8/Myostatin, TGF-beta, and WISP-1 (3, 5 ‑ 8). It also binds and activates EGF R, ErbB4, and IGF-I R (9 ‑ 11). In vivo, Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression (12 ‑ 15). Decorin is cleared from the extracellular space by LRP‑mediated endocytosis (16).

Item	Value
References	Schaefer, L. and R.V. Iozzo (2008) J. Biol. Chem. 283:21305. Scholzen, T. et al. (1994) J. Biol. Chem. 269:28270. Zamfir, A. et al. (2003) Glycobiology 13:733. Roughley, P.J. and R.J. White (1989) Biochem. J. 262:823. Ferdous, Z. et al. (2007) J. Biol. Chem. 282:35887. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675. Cabello-Verrugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842. Desnoyers, L. et al. (2001) J. Biol. Chem. 276:47599. Zhu, J.-X. et al. (2005) J. Biol. Chem. 280:32468. Santra, M. et al. (2000) J. Biol. Chem. 275:35153. Schonherr, E. et al. (2005) J. Biol. Chem. 280:15767. Olguin, H.C. et al. (2003) Dev. Biol. 259:209. Schonherr, E. et al. (2004) J. Vasc. Res. 41:499. Seidler, D.G. et al. (2006) J. Biol. Chem. 281:26408. Reed, C.C. et al. (2005) Oncogene 24:1104. Brandan, E. et al. (2006) J. Biol. Chem. 281:31562.
Entrez Gene IDs	1634 (Human); 13179 (Mouse)
Alternate Names	Bone proteoglycan II, CSCD, DCN, DSPG2, Decorin, PG-II, PG-S2, PG40, PGII, PGS2, SLRR1B, SLRR1BPGS2, decorin proteoglycan, dermatan sulphate proteoglycans II, proteoglycan core protein, small leucine-rich protein 1B

Citations for Recombinant Mouse Decorin Protein, CF (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Decorin Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Liu et al. (2021), DCN released from ferroptotic cells ignites AGER-dependent immune responses Autophagy, 0(0):1-14.
PMID: 34964698
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

J Chatterjee et al. (2021), Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia Nature Communications, 12(1):7122.
PMID: 34880260
AC Daquinag et al. (2020), Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly Cells, 9(12).
PMID: 33317052
Species: Mouse
Sample Types: Reference Standard

NMP Cartee et al. (2020), Binding of omeprazole to protein targets identified by monoclonal antibodies PLoS ONE, 15(9):e0239464.
PMID: 32946534
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

M Zhao et al. (2019), Mineralocorticoid receptor antagonism limits experimental choroidal neovascularization and structural changes associated with neovascular age-related macular degeneration Nat Commun, 10(1):369.
PMID: 30664640
Species: Mouse
Sample Types: Protein
Applications: Western Blot

AC Daquinag et al. (2017), Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells Mol Ther Oncolytics, 6(0):1-9.
PMID: 28607949
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Sneddon et al. (2012), Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature, 491(7426):765-8.
PMID: 23041930
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ichii et al. (2012), The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells. Blood, 119(7):1683-92.
PMID: 22117039
Species: Mouse
Sample Types: Recombinant Protein
Applications: Binding Assay

Daquinag AC et al. (2011), An isoform of decorin is a resistin receptor on the surface of adipose progenitor cells. Cell Stem Cell, 9(1):74-86.
PMID: 21683670

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