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Recombinant Mouse Cystatin B Protein, CF

Catalog # 1409-PI | R&D Systems, Inc. a Bio-Techne Brand
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1409-PI-050

Key Product Details

Source

E. coli

Accession #

Structure / Form

Monomer and a disulfide-linked multimer

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

E. coli-derived mouse Cystatin B protein
Met1-Phe98, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

12 kDa (monomer)

SDS-PAGE

13 kDa, reducing conditions

Activity

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog # ES009).
The IC50 value is <15 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1409-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM Tris and 100 mM NaCl, pH 7.5.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin B

Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Cystatin B-deficient mice have increased expression of proteolysis, apoptosis and glial activation genes, which is consistent with the pathology found in the mouse model of human progressive myoclonus epilepsy (EPM1) (3). The mouse Cystatin B consists of 98 amino acid residues (4).

References

  1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  2. Pol, E. and I. Bjork (1999) Biochemistry 38:10519.
  3. Lieuallen, K. et al. (2001) Hum. Mol. Genet. 10:1867.
  4. Pennacchio, L.A. and R.M. Myers (1996) Genome Res. 6:1103.

Alternate Names

CSTB, Stefin B

Entrez Gene IDs

1476 (Human); 13014 (Mouse)

Gene Symbol

CSTB

UniProt

Product Documents for Recombinant Mouse Cystatin B Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Cystatin B Protein, CF

For research use only

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