Recombinant Mouse Coagulation Factor III/Tissue Factor, CF

Name: Recombinant Mouse Coagulation Factor III/Tissue Factor, CF
Brand: R&D Systems
SKU: 3178-PA
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3178-PA

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(1)

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Key Product Details

Source:	NS0
Accession #:	P20352
Applications:	Enzyme Activity

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Product Specifications for Recombinant Mouse Coagulation Factor III/Tissue Factor, CF

Source	Mouse myeloma cell line, NS0-derived mouse Coagulation Factor III/Tissue Factor protein Ala29-Glu251, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala29
Predicted Molecular Mass	26 kDa
SDS-PAGE	38 kDa and 44 kDa, reducing conditions
Activity	Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011). The AC₅₀ is <5 µg/mL, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3178-PA

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
Reconstitution	Reconstitute at 200 μg/mL in sterile 50 mM Tris, pH 9.0.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1 to 28) and the mature chain (residues 29 to 294). As a type I membrane protein, it contains a transmembrane region (residues 252 to 274) and a cytoplasmic tail (residues 275 to 294) (3, 4). The purified rmTF corresponds to the ectodomain (residues 29 to 251) and is potent in activating thermolysin-processed Recombinant Mouse Coagulation Factor VII (Catalog # 3305-SE) under the conditions described in the Activity Assay Protocol.

Item	Value
References	Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496. Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.
Entrez Gene IDs	2152 (Human); 14066 (Mouse)
Alternate Names	CD142, CD142 antigen, Coagulation Factor III, F3, FLJ17960, TF, TFA, Thromboplastin, Tissue Factor, coagulation factor III (thromboplastin, tissue factor)

Citations for Recombinant Mouse Coagulation Factor III/Tissue Factor, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Coagulation Factor III/Tissue Factor, CF include:

Species: Mouse
Sample Types: Complex Sample Type
Applications: Bioassay

Y Wang et al. (2017), Rac1 regulates sepsis-induced formation of platelet-derived microparticles and thrombin generation Biochem. Biophys. Res. Commun., 0(0).
PMID: 28465231
Species: Mouse
Sample Types: Buffer
Applications: Enzyme Assay

Fernando S et al. (2009), Targeting tumor endothelial marker 8 in the tumor vasculature of colorectal carcinomas in mice. Cancer Res., 69(12):5126-32.
PMID: 19528090

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Customer Reviews for Recombinant Mouse Coagulation Factor III/Tissue Factor, CF (1)

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Recombinant Mouse Coagulation Factor III/Tissue Factor, CF

Name: Anonymous

Application: CellProlif

Reason for Rating: Tested the effect of TF on EC proliferation (red) in mice in vivo.

Verified Customer | Posted 12/05/2021

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Recombinant Mouse Coagulation Factor III/Tissue Factor, CF

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Product Specifications for Recombinant Mouse Coagulation Factor III/Tissue Factor, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Coagulation Factor III/Tissue Factor

Citations for Recombinant Mouse Coagulation Factor III/Tissue Factor, CF (2)

Customer Reviews for Recombinant Mouse Coagulation Factor III/Tissue Factor, CF (1)

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Recombinant Mouse Coagulation Factor III/Tissue Factor, CF

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