Recombinant Mouse CMG-2/ANTXR2 Fc Chimera Protein, CF

Name: Recombinant Mouse CMG-2/ANTXR2 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10418-AR

Catalog # 10418-AR

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	Q6DFX2.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse CMG-2/ANTXR2 Fc Chimera Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CMG-2/ANTXR2 protein

Mouse CMG-2/ANTXR2 (Gln32-Gly318) Accession # Q6DFX2.1	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

No results obtained. Gln32 inferred from enzymatic pyroglutamate treatment revealing Ala33.

Predicted Molecular Mass

58 kDa

SDS-PAGE

55-70 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Anthrax Protective Antigen is immobilized at 1.5 μg/mL (100 μL/well), it binds Recombinant Mouse CMG‑2/ANTXR2 Fc Chimera (Catalog # 10418-AR) with an ED₅₀ of 1-5 ng/mL.

Recombinant Mouse CMG-2/ANTXR2 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Mouse CMG-2/ANTXR2 Fc Chimera Protein Binding Activity When Anthrax Protective Antigen is immobilized at 1.5 μg/mL (100 μL/well), Recombinant Mouse CMG-2/ANTXR2 Fc Chimera (Catalog # 10418-AR) binds with an ED₅₀ of 1-5 ng/mL.
	Recombinant Mouse CMG-2/ANTXR2 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Mouse CMG-2/ANTXR2 Fc Chimera (Catalog # 10418-AR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands 55-70 kDa and 110-140 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10418-AR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: CMG-2/ANTXR2

Capillary Morphogenesis Gene-2 (CMG-2), also known as ANTXR2, is a widely expressed anthrax toxin receptor (ATR) family protein (1‑3). Mature, full-length CMG‑2/ANTXR2 is a type I transmembrane protein containing a von Willebrand A (vWA) domain in the extracellular domain (ECD) (1-5). In addition to the full-length protein, 3 additional isoforms exist: Isoform 2 shows a 103 amino acid (aa) deletion in the ECD; isoform 3 is a truncated, 289 aa soluble form; and isoforms 4 shows a 12 aa insertion in the cytoplasmic region (6). The ECD of mature, full-length mouse CMG‑2/ANTXR2 shares 84% and 89% aa sequence identity with human and rat CMG‑2/ANTXR2, respectively. The vWA domain in the ECD of CMG‑2/ANTXR2 adheres selectively to collagen type IV and laminin (1‑5). CMG-2/ANTXR2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (3). CMG‑2/ANTXR2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (7). CMG‑2/ANTXR2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus Anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (5, 8, 9). Transport to low pH endosomes, which requires CMG‑2/ANTXR2 ubiquitination and interaction with the LDL receptor related protein LRP6 (10, 11), allows PA pore formation and release of toxin to the cytoplasm (11, 12). Soluble CMG‑2/ANTXR2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). CMG‑2/ANTXR2 has been shown to interact with LRP6 and activate Wnt/ beta -catenin pathway in gastric cancer, which leads to maintenance of stem-like cells in GC (13).

Item	Value
References	Scobie, H.M. and J.A.T. Young (2005) Curr. Opin. Microbiol. 8:106. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170. Bell, S.E. et al. (2001) J. Cell Sci. 114:2755. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367. Santelli, E. et al. (2004) Nature 430:905. Cryan, L. et al. (2011) Front Biosci. 16:1574. Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957. Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349. Go, M.Y. et al. (2006) J. Mol. Biol. 360:145. Abrami, L. et al. (2006) J. Cell Biol. 172:309. Wei, W. et al. (2006) Cell 124:1141. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.
Long Name	Capillary Morphogenesis Protein 2
Entrez Gene IDs	118429 (Human); 71914 (Mouse)
Alternate Names	ANTXR2, CMG-2, CMG-2MGC45856, CMG2, CMG2MGC111533, Capillary morphogenesis gene 2 protein, FLJ31074, ISH, JHF, JHS, anthrax toxin receptor 2, cI-35, capillary morphogenesis protein 2

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