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Recombinant Mouse CLEC4B2 Fc Chimera Protein, CF

Catalog # 10087-DC | R&D Systems, Inc. a Bio-Techne Brand
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10087-DC-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CLEC4B2/mDCAR1 protein
MDP Mouse IgG2A
(Glu98-Lys330)
IEGR Mouse CLEC4B2
(Gln42-Leu208)
Accession # Q67DU8
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

47 kDa

SDS-PAGE

50-59 kDa, reducing conditions

Activity

Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
The ED50 for this effect is 0.25-1.25 μg/mL.

Scientific Data Images for Recombinant Mouse CLEC4B2 Fc Chimera Protein, CF

Recombinant Mouse CLEC4B2 Fc Chimera Protein Bioactivity

Recombinant Mouse CLEC4B2 Fc Chimera Protein Bioactivity

Recombinant Mouse CLEC4B2 Fc Chimera (Catalog # 10087-DC) binds fluorescein-conjugated S. aureus bioparticles. The ED50 for this effect is 0.25-1.25 μg/mL.

Formulation, Preparation and Storage

10087-DC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC4B2/mDCAR1

CLEC4B2 (C-type Lectin domain family 4, member b2), also called dendritic cell immunoactivating receptor 1 (DCAR1) and antigen presenting cell lectin-like receptor A1 (APLRA1), is a type II membrane protein belonging to the cell surface C-type lectin domain family (1, 2). Mouse CLEC4B2 is synthesized as a 208 amino acid (aa) precursor that contains a 22 aa transmembrane segment and a 167 aa extracellular domain (ECD) containing a C-type lectin domain. The ECD of mouse CLEC4B2 shares 71% and 50% aa identity with rat CLEC4B2 ECD and human CLEC4B2 ECD, respectively. Activation of CLEC4B2 in the epidermis regulates immune defenses. CLEC4B2 potentially has a second role in host defense. CLEC4B2 is specifically required for the innate immune response to fungal infection and wounding and place this GPCR further upstream than any other known component of innate immune recognition and signaling in C. elegans epidermis.

References

  1. Flornes, L. M. et al. (2004) Immunogenetics 56:506.
  2. Kaden, S. A. et al. (2009) J. Immunol. 183:5069.
  3. Zugasti, O. et al. (2014) Nat. Immunol. 15:833.

Long Name

C-type lectin domain family 4, Member b2

Alternate Names

mDCAR1

Entrez Gene IDs

381809 (Mouse)

Gene Symbol

CLEC4B2

UniProt

Product Documents for Recombinant Mouse CLEC4B2 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse CLEC4B2 Fc Chimera Protein, CF

For research use only

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