Mouse myeloma cell line, NS0-derived mouse Chondroadherin protein Ala21-Thr349, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
No results obtained. Protein identity confirmed via Mass Spectrometry.
Predicted Molecular Mass
33-38 kDa, reducing conditions
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.08-048 μg/mL
Recombinant Mouse Chondroadherin His-tag Protein, CF Scientific Data Examples
Recombinant Mouse Chondroadherin His-tag Protein Bioactivity
Recombinant Mouse Chondroadherin (Catalog # 10033-CH) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.08-0.48 μg/mL.
Recombinant Mouse Chondroadherin His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse Chondroadherin was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 33-38 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
12 months from date of receipt, ≤ -20 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, ≤ -20 °C under sterile conditions after reconstitution.
Chondroadherin (CHAD), also known as SLRR4A, is a 38 kDa secreted protein belonging to the small leucine-rich proteoglycans (SLRPs) that help regulate the assembly and function of the extracellular matrix (ECM) (1). CHAD is highly expressed in cartilaginous tissues, with lower expression levels found in bone, tendon, and eye (2-4). Mature mouse CHAD is a 338 amino acid (aa) protein that contains twelve leucine-rich repeats (LRRs) including ten tandem leucine-rich repeats as well as N-terminal and C-terminal leucine-rich domains flanked by cysteine-rich regions. CHAD interacts with collagen II and mediates signaling between chondrocytes and the ECM by binding to the alpha 2 beta 1 integrin, heparan sulphate, and to cell surface proteoglycans like syndecans (5-9). In addition, CHAD also interacts with both N- and C-terminal globular domains of type VI collagen (10). Mouse CHAD shares 95% and 98% aa sequence identity with human and rat CHAD, respectively.
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Larson, T. et al. (1991) J. Biol. Chem. 266:20428.
Mizuno, M. et al. (1996) Calcif. Tissue Int. 59:163.
Johnson, J.M. et al. (2006) Mol. Vis. 12:1057.
Månsson, B. et al. (2001) J. Biol. Chem. 276:32883.
Paracuellos, P. et al. (2017) Matrix Biol. 63:106.
Batista, M. et al. (2014) Matrix Biol. 38:84.
Camper, L. et al. (1997) J. Cell Biol. 138:1159.
Haglund, L. et al. (2013) J. Biol. Chem. 288:995.
Wiberg, C. et al. (2002) J. Biol. Chem. 277:49120.
Which Brands are Currently Available on bio-techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments and Simple Plex Assays are available to quote. ProteinSimple branded instrument consumables and ACD branded products will be available on bio-techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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