Product Specifications for Recombinant Mouse CD2F-10/SLAMF9 Protein, CF
Mouse myeloma cell line, NS0-derived mouse CD2F-10/SLAMF9 protein Phe18-Leu230, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
36-60 kDa, reducing conditions
Meassure by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3+ T cells. The ED50 for this effect is 0.4‑2 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
The CD2 family of receptors is a subset of the immunoglobulin superfamily of glycoproteins. The CD2F‑10 protein, also known as SF2001 and CD85‑H1, was identified as a CD2 subfamily member using genome sequence data. Based on its structural organization, CD2F‑10 belongs to the SLAM (Signaling Lymphocyte Activation Molecule) subfamily of CD2 receptors and is designated SLAMF9. SLAM proteins function as co‑receptors for lymphocyte activation and/or adhesion (1). Mature mouse CD2F‑10 is a type I transmembrane glycoprotein that consists of a 215 amino acid (aa) extracellular domain (ECD) with one Ig‑like V‑set and one Ig‑like C2‑set domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2 ‑ 4). Unlike other SLAM proteins, the cytoplasmic domain of CD2F‑10 does not contain consensus immunoreceptor tyrosine‑based switch motifs (ITSMs) that mediate interactions with the signal transduction proteins SAP or EAT‑2 (1). Within the ECD, mouse CD2F‑10 shares 58% and 83% aa sequence identity with human and rat CD2F‑10, respectively. It shares 21% ‑ 38% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD48, CD84, CD229, CRACC, NTB‑A, and SLAM. CD2F‑10 is expressed in hematopoietic tissues and cell lines (3). R&D in house data indicate that CD2F‑10 costimulates the secretion of IFN‑ gamma from activated mouse T cells.
Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
Fennelly, J.A. et al. (2001) Immunogenetics 53:599.
Zhang, W. et al. (2001) Clin. Cancer Res. 7:822s.
Fraser, C.C. et al. (2002) Immunogenetics 53:843.
CD2 Family 10/SLAM Family Member 9
Entrez Gene IDs
89886 (Human); 98365 (Mouse)
CD2 family member 10, CD2F-10, CD2F-10MGC88194, CD2F10, CD84 homolog 1, CD84-H1, CD84-H1cluster of differentiation 2 antigen family member 10, CD84H1, SF2001, SLAM family member 9, SLAMF9, cluster of differentiation 84 homolog 1, signaling lymphocytic activation molecule family member 2001, signaling lymphocytic activation molecule family member 9
Citations for Recombinant Mouse CD2F-10/SLAMF9 Protein, CF
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Product Documents for Recombinant Mouse CD2F-10/SLAMF9 Protein, CF
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