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Recombinant Mouse CD200R1L Fc Chimera Protein, CF

Catalog # 10198-CD | R&D Systems, Inc. a Bio-Techne Brand
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10198-CD-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD200R1L protein
Mouse CD200R1L
(Ile25-Thr220)
Accession # Q6XJV6.1
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile25

Predicted Molecular Mass

48 kDa

SDS-PAGE

65-85 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse CD200R1L Fc Chimera (Catalog # 10198-CD) binds with an ED50 of 0.35-2.8 µg/mL.

Scientific Data Images for Recombinant Mouse CD200R1L Fc Chimera Protein, CF

Recombinant Mouse CD200R1L Fc Chimera Protein Binding Activity

Recombinant Mouse CD200R1L Fc Chimera Protein Binding Activity

When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse CD200R1L Fc Chimera (Catalog # 10198-CD) binds with an ED50 of 0.35-2.8 µg/mL.
Recombinant Mouse CD200R1L Fc Chimera Protein SDS-PAGE

Recombinant Mouse CD200R1L Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse CD200R1L Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 65-85 kDa and 130-170 kDa, respectively.

Formulation, Preparation and Storage

10198-CD
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: CD200R1L

CD200 Receptor 2 (CD200R2), or CD200R1L, is a type I transmembrane receptor for the CD200 cell surface glycoprotein. Mature CD200R2 consists of an extracellular domain (ECD) containing an Ig-like V-type domain and an Ig-like C2-type domain, a transmembrane segment and a short cytoplasmic domain. The ECD of mouse CD200R2 shares a 76% and 63% amino acid (aa) identity with the rat and human ECD, respectively. CD200R2 is strongly expressed on resting mast cells but deceased upon activation via Fc epsilonR1. It is also observed on monocytes, bone marrow-derived dendritic cells and TH2 cells (1, 2, 4). It has been demonstrated CD200R2 can interact with CD200 (3) and signal through adaptor protein, DAP12, via its lysine residue in the transmembrane region (2). In addition, it has been reported CD200 and CD200R2 interaction alters dendritic cell differentiation and enhances induction of CD4+CD25+Foxp3+ regulatory T cells in mouse transplant model (5).

References

  1. Wright, G. et al. (2003) J. Immunol. 171:3034.
  2. Voehringer, D. (2004) J. Biol. Chem. 279(52):54117.
  3. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  4. Minas, K. (2006) Crit Rev Immunol 26(3):213.
  5. Gorczynski, R. et al. (2008) J. Immunol. 180(9):5946.

Long Name

CD200 Receptor 1 Like

Alternate Names

CD200R2, CD200RLa

Entrez Gene IDs

344807 (Human); 271375 (Mouse); 501779 (Rat)

Gene Symbol

CD200R1L

UniProt

Product Documents for Recombinant Mouse CD200R1L Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse CD200R1L Fc Chimera Protein, CF

For research use only

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