Recombinant Mouse CD155/PVR His-tag Protein, CF

Name: Recombinant Mouse CD155/PVR His-tag Protein, CF
Brand: rnd
SKU: 6909-CD

Catalog # 6909-CD

Citations (1)

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Key Product Details

Source:	NS0
Accession #:	NP_081790
Applications:	Bioactivity

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Product Specifications

Source	Mouse myeloma cell line, NS0-derived mouse CD155/PVR protein Met1-Leu348, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asp29
Predicted Molecular Mass	35.6 kDa
SDS-PAGE	63‑80 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD155/PVR is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD96 (Catalog # 5690‑CD) that produces 50% optimal binding response is found to be approximately 0.015‑0.075 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6909-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70 kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). CD155 binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically (3). Mature mouse CD155 consists of a 318 amino acid (aa) extracellular domain (ECD) with one N-terminal V-type and two C2-type Ig-like domains, a 24 aa transmembrane segment, and a 38 aa cytoplasmic tail. Within the ECD, mouse CD155 shares 46%, 73%, and 44% aa sequence identity with human CD155, rat CD155, and mouse Nectin-2, respectively. The V-type domain of CD155 mediates all binding, including to polio virus (1), and alternative splicing within this domain in humans can modulate ligand binding (4). Human CD155 can also be spliced to generate secreted isoforms (5). CD155 is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 6-9). It is preferentially expressed on Th17 cells compared to Th2 cells (10), and its activation promotes the development of Th1 responses (11). On migrating cells, CD155 is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits
Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration (1, 3, 12). Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM-1 or CD96 expressing NK cells (1, 7, 13). Binding of monocyte DNAM-1 to endothelial cell CD155 promotes transendothelial migration (8). The expression of CD155 on mouse CD8+ thymocytes prevents their premature exit from the thymus (14). Within intestinal Peyer's patches, follicular dendritic cell CD155 activates follicular helper T cells via DNAM-1 or CD96 binding (7-9, 15). CD155 also binds the inhibitory ligand TIGIT on NK and some mature T cells, antagonizing DNAM-1 effects (7, 15, 16).

Item	Value
References	Mandai, K. et al. (2015) Curr. Top. Dev. Biol. 112:197. Ravens, I. et al. (2003) Biochem. Biophys. Res. Commun. 312:1364. Sato, T. et al. (2004) Genes to Cells 9:791. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235. Koike, S. et al. (1990) EMBO J. 9:3217. Escalante, N.K. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:1177. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11. Reymond, N. et al. (2004) J. Exp. Med. 199:1331. Maier, M.K. et al. (2007) Eur. J. Immunol. 37 :2214. Lozano, E. et al. (2013) J. Immunol. 191:3673. Yamashita-Kanemaru, Y. et al. (2015) J. Immunol. 194:5644. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251. Chan, C.J. et al. (2010) J. Immunol. 184:902. Qui, Q. et al. (2010) J. Immunol. 184:1681. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
Long Name	Poliovirus Receptor
Entrez Gene IDs	5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)
Alternate Names	CD155, CD155 antigen, HVED, NECL5, Necl-5, Nectin-like protein 5, PVR, PVS, PVSFLJ25946, Tage4, nectin-like 5, poliovirus receptor

Citations (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse CD155/PVR His-tag Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Bellucci R et al. (2012), Tyrosine kinase pathways modulate tumor susceptibility to natural killer cells. J. Clin. Invest., 122(7):2369-83.
PMID: 22684105

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Recombinant Mouse CD155/PVR His-tag Protein, CF

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Key Product Details

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Product Specifications

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CD155/PVR

Citations (1)

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