Recombinant Mouse CCL9/10/MIP-1 gamma Protein

Name: Recombinant Mouse CCL9/10/MIP-1 gamma Protein
Brand: R&D Systems
SKU: 463-MG
Price: 351 USD

Catalog # 463-MG | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

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Source:	E. coli
Accession #:	Q3U9T8
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse CCL9/10/MIP-1 gamma Protein

Source	E. coli-derived mouse CCL9/10/MIP-1 gamma protein Gln22-Gln122
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gln22
Predicted Molecular Mass	11.5 kDa
Activity	Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 0.2-0.6 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED₅₀ for this effect is 0.002-0.01 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

463-MG

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

463-MG/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL9/10/MIP-1 gamma

Mouse CCL9/10 (also named MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1 - 3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3 - 7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71 - 73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full-length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).

Item	Value
References	Zlotnik, A. and O. Yoshie (2000) Immunity 12:121. Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1 Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102. Haelens, A. et al. (1996) Immunobiology 195:499. Berahovich, R.D. et al. (2005) J. Immunol. 174:7341. Youn, B-S. et al. (1995) J. Immunol. 155:2661. Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.
Entrez Gene IDs	20308 (Mouse)
Alternate Names	CCF18, CCL9/10, MIP-1 gamma, MIP1 gamma

Citations for Recombinant Mouse CCL9/10/MIP-1 gamma Protein (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse CCL9/10/MIP-1 gamma Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

L Li et al. (2020-02-25), Astroglial TLR9 antagonism promotes chemotaxis and alternative activation of macrophages via modulation of astrocyte-derived signals: implications for spinal cord injury J Neuroinflammation, 2020-017(1):73.
PMID: 32098620
Species: Mouse

Applications: Binding Assay

S Sharif et al. (2019-06-25), Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 2019-010(0):1421.
PMID: 31293551
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Binder NB et al. (2009-03-29), Estrogen-dependent and C-C chemokine receptor-2-dependent pathways determine osteoclast behavior in osteoporosis. Nat. Med., 2009-015(4):417-24.
PMID: 19330010
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Okamatsu Y et al. (2004-08-01), MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival. J. Immunol., 2004-0173(3):2084-90.
PMID: 15265944

Recombinant Mouse CCL9/10/MIP-1 gamma Protein

Catalog # 463-MG | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse CCL9/10/MIP-1 gamma Protein

Formulation, Preparation and Storage

Carrier Free

463-MG

Carrier

463-MG/CF

Carrier Free

Reconstitution Calculator

Background: CCL9/10/MIP-1 gamma

Citations for Recombinant Mouse CCL9/10/MIP-1 gamma Protein (4)

Product Documents for Recombinant Mouse CCL9/10/MIP-1 gamma Protein

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