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Recombinant Mouse Cathepsin H Protein, CF

Catalog # 1013-CY | R&D Systems, Inc. a Bio-Techne Brand
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1013-CY-010

Key Product Details

Source

NS0

Accession #

Structure / Form

Pro form

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Cathepsin H protein
Glu22-Val333, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu22

Predicted Molecular Mass

36 kDa

SDS-PAGE

40 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC).
The specific activity is >400 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com

Formulation, Preparation and Storage

1013-CY
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Cathepsin H

Cathepsin H is a lysosomal cysteine protease of the papain family (1). It is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑95), a mini chain (residues 96‑103), a heavy chain (residues 114‑290) and a light chain (residues 291‑333) (2, 3). A truncated form with a 12 amino acid deletion in the signal peptide region is secreted (4). Cathepsin H is the only known mono-aminopeptidase in the papain family (5). Cathepsin H expression is significantly increased in disease states such as in prostate tumors, sera of asthmatic patients, and mucosa of colorectal cancer patients (4, 6, 7).

References

  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1089, Academic Press, San Diego. 
  2. Lafuse, W.P. et al. (1995) J. Leukoc. Biol. 57:663.
  3. Soderstrom, M. et al. (1999) Biochim. Biophys. Acta 1446:35.
  4. Waghray, A. et al. (2002) J. Biol. Chem. 277:11533.
  5. Guncar, G. et al. (1998) Structure 6:51.
  6. Cimerman, N. et al. (2001) Clin. Chim. Acta 310:113.
  7. del Re, E.C. et al. (2000) Br. J. Cancer. 82:1317.

Alternate Names

CTSH

Entrez Gene IDs

1512 (Human); 13036 (Mouse)

Gene Symbol

CTSH

UniProt

Product Documents for Recombinant Mouse Cathepsin H Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Cathepsin H Protein, CF

For research use only

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