Source	Mouse myeloma cell line, NS0-derived mouse Cathepsin H protein Glu22-Val333, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu22
Predicted Molecular Mass	36 kDa
SDS-PAGE	40 kDa, reducing conditions
Activity	Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC). The specific activity is >400 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1013-CY

Formulation	Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

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Background: Cathepsin H

Cathepsin H is a lysosomal cysteine protease of the papain family (1). It is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑95), a mini chain (residues 96‑103), a heavy chain (residues 114‑290) and a light chain (residues 291‑333) (2, 3). A truncated form with a 12 amino acid deletion in the signal peptide region is secreted (4). Cathepsin H is the only known mono-aminopeptidase in the papain family (5). Cathepsin H expression is significantly increased in disease states such as in prostate tumors, sera of asthmatic patients, and mucosa of colorectal cancer patients (4, 6, 7).

Item	Value
References	Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1089, Academic Press, San Diego. Lafuse, W.P. et al. (1995) J. Leukoc. Biol. 57:663. Soderstrom, M. et al. (1999) Biochim. Biophys. Acta 1446:35. Waghray, A. et al. (2002) J. Biol. Chem. 277:11533. Guncar, G. et al. (1998) Structure 6:51. Cimerman, N. et al. (2001) Clin. Chim. Acta 310:113. del Re, E.C. et al. (2000) Br. J. Cancer. 82:1317.
Entrez Gene IDs	1512 (Human); 13036 (Mouse)
Alternate Names	ACC-4, ACC-5, CPSB, CTSH, Cathepsin H, DKFZp686B24257, EC 3.4.22, EC 3.4.22.16, MGC1519, N-benzoylarginine-beta-naphthylamide hydrolase, aleurain, cathepsin B3, cathepsin BA, minichain

Citations for Recombinant Mouse Cathepsin H Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Cathepsin H Protein, CF include:

Species: Human
Sample Types: Cell Lysates
Applications: Enzyme Assay

Burster T et al. (2007), Design of protease-resistant myelin basic protein-derived peptides by cleavage site directed amino acid substitutions. Biochem. Pharmacol., 74(10):1514-23.
PMID: 17803968

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Recombinant Mouse Cathepsin H Protein, CF

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