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Recombinant Mouse Cathepsin E Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1130-AS

Catalog #
Size / Price

Key Product Details



Accession #

Structure / Form

Disulfide-linked homodimer




Enzyme Activity

Product Specifications


Mouse myeloma cell line, NS0-derived mouse Cathepsin E protein
Gln19-Pro397, with a C-terminal 10-His tag


>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr55 & Gln19 (predicted)

Predicted Molecular Mass

42 & 38 kDa


53 kDa, reducing conditions


Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The specific activity is >8,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Cathepsin E

Cathepsin E is an intracellular aspartic protease of the pepsin family (1, 2). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsins D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (3). Both enzymes are efficient in cleaving the Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with the wild-type APP (4). Mouse Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑18), a propeptide (residues 19‑59), and a mature chain (residues 60‑397) (1).


  1. Tatnell, P.J. et al. (1997) FEBS Lett. 408:62.
  2. Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.), p. 33,  Academic Press, San Diego.
  3. Tsukuba, T. et al. (2000) Mol. Cells 10:601.
  4. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.

Alternate Names


Entrez Gene IDs

1510 (Human); 13034 (Mouse)

Gene Symbol



Additional Cathepsin E Products

Product Documents for Recombinant Mouse Cathepsin E Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Cathepsin E Protein, CF

For research use only