Recombinant Mouse Cathepsin C/DPPI Protein, CF

Name: Recombinant Mouse Cathepsin C/DPPI Protein, CF
Brand: R&D Systems
SKU: 1034-CY

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1034-CY

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	P97821
Structure / Form:	Pro form
Applications:	Enzyme Activity

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Product Specifications for Recombinant Mouse Cathepsin C/DPPI Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse Cathepsin C/DPPI protein Asp25-Leu462, with a C-terminal 10-His tag Accession # P97821
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asp25
Predicted Molecular Mass	51 kDa
SDS-PAGE	52 kDa, reducing conditions
Activity	Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Arg-7-amido-4-methylcoumarin (GR-AMC). The specific activity is >8,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1034-CY

Formulation	Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: Cathepsin C/DPPI

Cathepsin C is a cysteine protease of the papain family (1). Cathepsin C sequentially removes dipeptides from the free N-termini of proteins and peptides. It has broad specificity except that it does not cleave a basic amino acid (Arg or Lys) in the N-terminal position or Pro on either side of the scissle bond. It requires halide ions for activity. The pro form contains a pro peptide and a catalytic region, which can be further processed into heavy/ alpha and light/ beta chains that are linked by a disulfide bond. It is broadly distributed. Cathepsin C plays a role in the lysosomal degradation. It also functions as a key enzyme in the activation of granule serine proteases in cytotoxic T lymphocytes and natural killer cells (granzymes A and B), mast cells (tryptase and chymase), and neutrophils (Cathepsin G and elastase) by removing their N-terminal activation dipeptides (2).

Item	Value
References	Turk, B. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, et al. eds. p. 1192, Academic Press, San Diego. Dahl, S.W. et al. (2001) Biochemistry 40:1671.
Entrez Gene IDs	1075 (Human); 13032 (Mouse); 25423 (Rat)
Alternate Names	CPPIHMS, CTSC, Cathepsin C, Cathepsin J, DPP-I, DPP1, DPPI, Dipeptidyl peptidase I, Dipeptidyl transferase, JP, JPD, PALS, PLS, cathepsin CEC 3.4.14.1, dipeptidyl peptidase 1, dipeptidyl-peptidase I

Citations for Recombinant Mouse Cathepsin C/DPPI Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Cathepsin C/DPPI Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

S Matthiesen et al. (2020), Coxiella burnetii-infected NK cells release infectious bacteria by degranulation Infect. Immun., 0(0).
PMID: 32817330
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Matthiesen et al. (2020), Coxiella burnetii-infected NK cells release infectious bacteria by degranulation Infect. Immun., 0(0).
PMID: 32817330

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