Product Specifications for Recombinant Mouse Caspr2 His-tag Protein, CF
Mouse myeloma cell line, NS0-derived mouse Caspr2 protein Ala28-Ser1262, with a C-terminal 6-His tag
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
130-150 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Caspr2 His-tag (Catalog # 10196-CR) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human Contactin-1 Fc Chimera (Catalog # 904-CN) that produces 50% of the optimal binding response is 5-30 μg/mL.
Scientific Data Examples for Recombinant Mouse Caspr2 His-tag Protein, CF
Recombinant Mouse Caspr2 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse Caspr2 His-tag (Catalog # 10196-CR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 130-150 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Contactin Associated Protein-Like 2 (CASPR2), also known as Cntnap2, is a neuronal type I transmembrane member of the neurexin family of adhesion molecules (ref). CASPR2, along with CASPR1 (or Cntnap1), is required for radical and longitudinal organization of myelinated axons (1). Mature mouse CASPR2 consists of a large extracellular domain (ECD) containing four Laminin G-like domains as well as two EFG-like domains and a Fibrinogen C-terminal domain, a transmembrane segment, and a short cytoplasmic domain. The ECD of mouse CASPR2 shares 94% and 93% amino acid (aa) identity with the human and rat ECD, respectively. CASPR2 is thought to be important for the control of neuron-glia interactions and is associated with alterations in synaptic structure, neuronal network activity, and an autism-related phenotype in mice (2, 3). In myelinated nerves, CASPR2 is confined to the juxtaparanodal region of the axon where it appears to associate with the immunoglobulin domains of TAG-1 (transient axonal glycoprotein 1) to form a scaffold, which clusters the potassium channels Kv1.1 and Kv1.2 (4). Recent studies demonstrate that CASPR2 binds to contactin1 (5).
Gordon A. et al. (2014) J. Neurosci. 10:1523.
Cope, E. et al. (2016) eNeuro. 0196-16.
Peñagarikano O. et al. (2011) Cell. 147(1):236.
Poliak, S. et al. (1999) Neuron 24:1037.
Rubio-Marrero, E. N. et al. (2016) J. Bio. Chem. 291:5788.
Contactin Associated Protein-like 2
Entrez Gene IDs
26047 (Human); 66797 (Mouse)
AUTS15, CASPR2DKFZp781D1846, CDFE, CNTNAP2, Caspr2, Cell recognition molecule Caspr2, KIAA0868, NRXN4, NRXN4Caspr2, PTHSL1, contactin associated protein-like 2, contactin-associated protein 2, contactin-associated protein-like 2, homolog of Drosophila neurexin IV
Citations for Recombinant Mouse Caspr2 His-tag Protein, CF
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Product Documents for Recombinant Mouse Caspr2 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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