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Recombinant Mouse Carboxypeptidase B1/CPB1 Protein, CF

Catalog # 2898-ZN | R&D Systems, Inc. a Bio-Techne Brand
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2898-ZN-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Carboxypeptidase B1/CPB1 protein
Ser16-Tyr415, with a C-terminal 10-His tag
Accession # NP_083982

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser16

Predicted Molecular Mass

47 kDa

SDS-PAGE

43 kDa and 48 kDa, reducing conditions

Activity

Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg.
The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

2898-ZN
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Carboxypeptidase B1/CPB1

Carboxypeptidase B1, encoded by the CPB1 gene, specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg (1). The deduced amino acid sequence of mouse CPB1 consists of a signal peptide (residues 1 to 15), a pro region (residue 16 to 108), and a mature chain (residues 109 to 415). The purified rmCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro (1).

References

  1. Aviles, F.X. and J. Vendrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 831, Academic Press, San Diego.

Alternate Names

CPB1, PASP, PCPB

Entrez Gene IDs

1360 (Human); 76703 (Mouse)

Gene Symbol

CPB1

UniProt

Product Documents for Recombinant Mouse Carboxypeptidase B1/CPB1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Carboxypeptidase B1/CPB1 Protein, CF

For research use only

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