Product Specifications for Recombinant Mouse BTN1A1/Butyrophilin Protein, CF
Mouse myeloma cell line, NS0-derived mouse BTN1A1/Butyrophilin protein Ala27-Trp247, with a C-terminal 6-His tag
>95%, by SDS-PAGE with silver staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
34-42 kDa, reducing conditions
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL.
Scientific Data Examples for Recombinant Mouse BTN1A1/Butyrophilin Protein, CF
Recombinant Mouse BTN1A1/Butyrophilin Protein Bioactivity
Recombinant Mouse BTN1A1/Butyrophilin (Catalog # 8540-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Butyrophilin 1A1 (also called BTN1A1), a 58 kDa type I transmembrane glycoprotein, is a member of the Ig superfamily. BTN1A1 is 498 amino acids (aa) long and is composed of an extracellular domain (ECD), a transmembrane domain and a cytoplasmic tail which contains the B30.2 domain. The BTN1A1 ECD displays two predicted IgV and IgC domains as do B7 and Skint proteins which interact with other Ig superfamily members (1).The B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR) (2). This interaction stabilizes the association of XOR with the milk fat globule membrane and appears to be essential in the control of milk fat globule secretion (3, 4, 5). Binding to XOR is conserved among BTN1A1 orthologs, but is not shared by BTN2A1 or BTN3A1 (2). The B30.2 domain of butyrophilins is also described as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations. B30.2 binding to pAg induces a cascade of events leading to the activation of gamma δ T cells (6). In vitro, BTN1A1 has an inhibitory effect on CD4+ T cell proliferation, and in addition reduces expression of cytokines associated with T cell activation such as IL-2 and IFN-gamma (7, 8). Furthermore, in vivo, BTN1A1 has a protective effect against the development of experimental autoimmune encephalomyelitis (EAE) (9). The ECD of mouse BTN1A1 shares 68% aa sequence identity with both human and rat BTN1A1. Because butyrophilins are structurally related to B7 proteins and are functionally implicated in immune regulation, they may represent an emerging family of co-stimulatory/inhibitory molecules.
Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
Jeong, J. et al. (2009) J. Biol. Chem. 284:22444.
Vorback, C. et al. (2002) Genes Dev. 16:3223.
Ogg, S.L. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10084.
Robenek, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10385.
Sandstrom, A. et al. (2014) Immunity 40:490.
Arnet, H.A. and Viney, J.L. (2014) Nat.Rev.Immunol.14:559.
Smith, I.A. et al. (2010) J.Immunol. 184:3514.
Mana, P. et al. (2004) J.Immunol. 16:489.
Entrez Gene IDs
696 (Human); 12231 (Mouse); 306956 (Rat)
BTBTNbK14H9.2 (butyrophilin, subfamily 1, member A1), BTN, BTN1A1, butyrophilin subfamily 1 member A1, butyrophilin, subfamily 1, member A1
Citations for Recombinant Mouse BTN1A1/Butyrophilin Protein, CF
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Product Documents for Recombinant Mouse BTN1A1/Butyrophilin Protein, CF
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