Recombinant Mouse BTN1A1/Butyrophilin Protein, CF

Name: Recombinant Mouse BTN1A1/Butyrophilin Protein, CF
Brand: R&D Systems
SKU: 8540-BT

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8540-BT

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Key Product Details

Source:	NS0
Accession #:	Q62556
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse BTN1A1/Butyrophilin Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse BTN1A1/Butyrophilin protein Ala27-Trp247, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE with silver staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala27
Predicted Molecular Mass	25 kDa
SDS-PAGE	34-42 kDa, reducing conditions
Activity	Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.3-1.5 μg/mL.

Scientific Data Examples for Recombinant Mouse BTN1A1/Butyrophilin Protein, CF

	Recombinant Mouse BTN1A1/Butyrophilin Protein Bioactivity Recombinant Mouse BTN1A1/Butyrophilin (Catalog # 8540-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.3-1.5 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8540-BT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: BTN1A1/Butyrophilin

Butyrophilin 1A1 (also called BTN1A1), a 58 kDa type I transmembrane glycoprotein, is a member of the Ig superfamily. BTN1A1 is 498 amino acids (aa) long and is composed of an extracellular domain (ECD), a transmembrane domain and a cytoplasmic tail which contains the B30.2 domain. The BTN1A1 ECD displays two predicted IgV and IgC domains as do B7 and Skint proteins which interact with other Ig superfamily members (1).The B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR) (2). This interaction stabilizes the association of XOR with the milk fat globule membrane and appears to be essential in the control of milk fat globule secretion (3, 4, 5). Binding to XOR is conserved among BTN1A1 orthologs, but is not shared by BTN2A1 or BTN3A1 (2). The B30.2 domain of butyrophilins is also described as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations. B30.2 binding to pAg induces a cascade of events leading to the activation of gamma δ T cells (6). In vitro, BTN1A1 has an inhibitory effect on CD4⁺ T cell proliferation, and in addition reduces expression of cytokines associated with T cell activation such as IL-2 and IFN-gamma (7, 8). Furthermore, in vivo, BTN1A1 has a protective effect against the development of experimental autoimmune encephalomyelitis (EAE) (9). The ECD of mouse BTN1A1 shares 68% aa sequence identity with both human and rat BTN1A1. Because butyrophilins are structurally related to B7 proteins and are functionally implicated in immune regulation, they may represent an emerging family of co-stimulatory/inhibitory molecules.

Item	Value
References	Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34. Jeong, J. et al. (2009) J. Biol. Chem. 284:22444. Vorback, C. et al. (2002) Genes Dev. 16:3223. Ogg, S.L. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10084. Robenek, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10385. Sandstrom, A. et al. (2014) Immunity 40:490. Arnet, H.A. and Viney, J.L. (2014) Nat.Rev.Immunol.14:559. Smith, I.A. et al. (2010) J.Immunol. 184:3514. Mana, P. et al. (2004) J.Immunol. 16:489.
Entrez Gene IDs	696 (Human); 12231 (Mouse); 306956 (Rat)
Alternate Names	BTBTNbK14H9.2 (butyrophilin, subfamily 1, member A1), BTN, BTN1A1, butyrophilin subfamily 1 member A1, butyrophilin, subfamily 1, member A1

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