Recombinant Mouse BMP-9 Protein

Name: Recombinant Mouse BMP-9 Protein
Brand: R&D Systems
SKU: 5566-BP
Price: 483 USD

Catalog # 5566-BP | R&D Systems, Inc. a Bio-Techne Brand

Citations (14)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	AAD56961
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse BMP-9 Protein

Product Specifications for Recombinant Mouse BMP-9 Protein

Source	Chinese Hamster Ovary cell line, CHO-derived mouse BMP-9 protein Ser319-Arg428
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser319
Predicted Molecular Mass	12.1 kDa
SDS-PAGE	13 kDa, reducing conditions
Activity	Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 0.5-4 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

5566-BP

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5566-BP/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: BMP-9

Bone morphogenetic protein 9 (BMP-9), also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP family of TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). Mouse BMP-9 consists of a 22 amino acid (aa) signal sequence, a 296 aa propeptide, and a 110 aa mature protein. Unlike other BMP family propeptides, the BMP-9 propeptide does not interfere with the biological activity of BMP-9, and remains associated with the mature peptide after proteolytic cleavage (3). Mouse and human BMP-9 share 96% aa sequence identity. Within the mature protein, mouse BMP-9 shares 64% aa sequence identity with mouse BMP-10 and approximately 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver where it promotes lipid metabolism and inhibits glucose production (4 - 6). It exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (6). BMP-9 interacts with the high affinity ALK-1 receptor on liver endothelial cells (3 - 5). It induces a cholinergic neuronal phenotype as well as the differentiation of mesenchymal stem cells into the chondrogenic lineage (7 - 11). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations it enhances TGF-beta 1 production, and inhibits hematopoietic progenitor colony formation (12). BMP-9 also inhibits angiogenesis and the invasiveness of prostate carcinoma cells (13, 14).

Item	Value
References	Chen, D. et al. (2004) Growth Factors 22:233. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111. Song, J.J. et al. (1995) Endocrinology 136:4293. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937. Chen, C. et al. (2003) Nat. Biotechnol. 21:294. Lopez-Coviella, I. et al. (2000) Science 289:313. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611. Ploemacher, R.E. et al. (1999) Leukemia 13:428. Ye, L. et al. (2008) Mol. Cancer Res. 6:1594. David, L. et al. (2008) Circ. Res. 102:914.
Long Name	Bone Morphogenetic Protein 9
Entrez Gene IDs	2658 (Human); 12165 (Mouse)
Alternate Names	BMP-9, BMP9, BMP9BMP-9Bone morphogenetic protein 9, GDF-2, GDF2, growth differentiation factor 2, growth/differentiation factor 2

Citations for Recombinant Mouse BMP-9 Protein (14)

Showing 1 - 10 of 14 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse BMP-9 Protein include:

Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay

T Ahmed et al. (2022-07-21), EPDR1 is a Non-canonical Effector of Insulin-mediated Angiogenesis Regulated by an Endothelial-specific TGF-beta Receptor Complex The Journal of Biological Chemistry, 2022-00(0):102297.
PMID: 35872017
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

T Yamamoto et al. (2022-06-20), Factors affecting the induction of uncoupling protein 1 in C2C12 myogenic cells Cytokine, 2022-0157(0):155936.
PMID: 35738051
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

DL Marvin et al. (2022-05-19), Dynamic Visualization of TGF-beta/SMAD3 Transcriptional Responses in Single Living Cells Cancers, 2022-014(10).
PMID: 35626109
Species: Feline
Sample Types: Whole Cells
Applications: Bioassay

M Chiara Per et al. (2021-03-28), Effects of selected hormones and their combination on progesterone and estradiol production and proliferation of feline granulosa cells cultured inï¿½vitro Theriogenology, 2021-0168(0):1-12.
PMID: 33826978
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

B Tao et al. (2020-10-23), BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells J Biol Chem, 2020-10(0).
PMID: 33097593
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Gadomski et al. (2020-04-28), Id1 and Id3 Maintain Steady-State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration Cell Rep, 2020-031(4):107572.
PMID: 32348770
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

H Gaitantzi et al. (2020-03-04), BMP-9 Modulates the Hepatic Responses to LPS Cells, 2020-09(3).
PMID: 32143367
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

YH Kim et al. (2018-11-06), SMAD4 Deficiency Leads to Development of Arteriovenous Malformations in Neonatal and Adult Mice J Am Heart Assoc, 2018-17(21):e009514.
PMID: 30571376
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Ouarné et al. (2018-08-30), BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer J. Exp. Clin. Cancer Res., 2018-037(1):209.
PMID: 30165893
Species: Mouse
Sample Types: Serum
Applications: ELISA (Capture)

(2016-11-22), A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10 Sci Rep, 2016-15(0):37366.
PMID: 27874028
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Alaa El Din F et al. (2015-07-15), Functional and splicing defect analysis of 23 ACVRL1 mutations in a cohort of patients affected by Hereditary Hemorrhagic Telangiectasia. PLoS ONE, 2015-010(7):e0132111.
PMID: 26176610
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Azuma K et al. (2015-07-01), Osteoblast-Specific gamma-Glutamyl Carboxylase-Deficient Mice Display Enhanced Bone Formation With Aberrant Mineralization. J Bone Miner Res, 2015-030(7):1245-54.
PMID: 25600070
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Zhu D et al. (2014-10-09), BMP-9 regulates the osteoblastic differentiation and calcification of vascular smooth muscle cells through an ALK1 mediated pathway. J Cell Mol Med, 2014-119(1):165-74.
PMID: 25297851
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Chen et al. (2012-12-02), H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs. Nat Genet, 2012-145(1):34-42.
PMID: 23202127

Recombinant Mouse BMP-9 Protein

Catalog # 5566-BP | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Mouse BMP-9 Protein

Formulation, Preparation and Storage

Carrier Free

5566-BP

Carrier

5566-BP/CF

Carrier Free

Reconstitution Calculator

Background: BMP-9

Citations for Recombinant Mouse BMP-9 Protein (14)

Product Documents for Recombinant Mouse BMP-9 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected