Recombinant Mouse Betacellulin Protein

Name: Recombinant Mouse Betacellulin Protein
Brand: rnd
SKU: 1025-CE

Catalog # 1025-CE

Citations (4)

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Key Product Details

Source:	E. coli
Accession #:	Q543J8
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Betacellulin Protein

Product Specifications

Source	E. coli-derived mouse Betacellulin/BTC protein Asp32-Gln118
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asp32
Predicted Molecular Mass	9.9 kDa
Activity	Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED₅₀ for this effect is 0.15-0.6 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1025-CE

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1025-CE/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Betacellulin/BTC

Betacellulin (BTC) is a member of the EGF family of cytokines which includes EGF, TGF-alpha, amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region (1). BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien (2). The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing (3). Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively (1). The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5 (4). BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum (5). BTC promotes pancreatic beta-cell growth and differentiation (6) and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (1). The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers (1).

Item	Value
References	Dunbar, A.J. and C. Goddard (2000) Int. J. Biochem. Cell Biol. 32:805. Shing, Y. et al. (1993) Science 259:1604. Tada, H. et al. (1999) J. Cell Biochem. 72:423. Pathak, B.G. et al. (1995) Genomics 28:116. Bastian, S.E. et al. (2001) J. Endocrinol. 168:203. Li, L. et al. (2001) Endocrinology 142:5379.
Entrez Gene IDs	685 (Human); 12223 (Mouse)
Alternate Names	BTC, Betacellulin, probetacellulin

Citations (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Betacellulin Protein include:

Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

LJ Martin et al. (2017), Epiregulin and EGFR interactions are involved in pain processing J. Clin. Invest., 0(0):.
PMID: 28783046
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Harada M et al. (2015), Temporal expression of growth factors triggered by epiregulin regulates inflammation development. J Immunol, 194(3):1039-46.
PMID: 25556244
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Yilmaz M et al. (2011), Transcription factor Dlx2 protects from TGFbeta-induced cell-cycle arrest and apoptosis. EMBO J., 30(21):4489-99.
PMID: 21897365
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cho YM et al. (2007), Betacellulin and nicotinamide sustain PDX1 expression and induce pancreatic beta-cell differentiation in human embryonic stem cells. Biochem. Biophys. Res. Commun., 366(1):129-34.
PMID: 18060872

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Product Documents

Product Datasheets

Download Product Datasheets: 1025-CE (with carrier)

Download Product Datasheets: 1025-CE/CF (carrier free)

Recombinant Mouse Betacellulin Protein

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Key Product Details

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Product Specifications

Formulation, Preparation and Storage

Carrier Free

1025-CE

Carrier

1025-CE/CF

Carrier Free

Reconstitution Calculator

Background: Betacellulin/BTC

Citations (4)

Product Documents

Product Datasheets

COA

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SDS

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