Product Specifications for Recombinant Mouse Ameloblastin Protein, CF
Mouse myeloma cell line, NS0-derived mouse Ameloblastin protein Val27-Pro422, with a C-terminal 6-His tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Val27 & Ala29
Predicted Molecular Mass
70-80 kDa, reducing conditions
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 1-4 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 250 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Ameloblastin (AMBN) is a secreted bone and tooth-specific glycoprotein member of the enamel matrix protein family. Mouse Ameloblastin is synthesized by ameloblasts and osteoblasts as a 407 amino acid (aa; short or “a” isoform) or 422 aa (long or “b” isoform) protein with predicted molecular masses of 44 kDa or 45 kDa, respectively (1). The long isoform includes an additional 15 aa insert following aa 103 and contains additional glycosylation sites (2). This recombinant protein corresponds to the mature long isoform of mouse Ameloblastin. Ameloblastin may be cleaved following secretion leading to the generation of multiple sized proteins from 17-66 kDa (3-6). Mouse Ameloblastin (long isoform) shares 60% and 96% aa sequence identity with the human and rat orthologs, respectively. Ameloblastin is a critical component of tooth enamel and bone matrix where it binds calcium, thereby facilitating bone and tooth mineralization. It has also been shown to self-associate into ribbon-like supramolecular structures (7). During tooth development, it acts by binding to and maintaining the differentiated phenotype of secretory ameloblasts (8, 9). During bone development, Ameloblastin regulates osteogenic differentiation by binding to CD63 and promoting CD63-Integrin beta 1 interactions (10). Ameloblastin may also promote osteoclastogenesis (11). Ameloblastin can act as an adhesion molecule and promotes the adhesion of periodontal ligament cells via RhoA activation and ameloblasts via heparin-binding domains (8, 12, 13).
Simmons, D. et al. (1998) Connect Tissue Res. 39:3.
Kobayashi, K. et al. (2007) J. Dent. Res. 86:962.
Uchida, T. et al. (1997) J. Histochem. Cytochem. 45:1329.
Chun, Y.H. et al. (2010) J. Dent. Res. 89:785.
Ravindranath, R.M. et al. (2007) J. Biol. Chem. 282:36370.
Yamakoshi, Y. et al. (2011) Eur. J. Oral Sci. 119 Suppl 1:206.
Wald, T. et al. (2013) J. Biol. Chem. 288:22333.
Fukumoto, S. et al. (2004) J. Cell Biol. 167:973.
Fukumoto, S. et al. (2005) Cells Tissues Organs 181:189.
Iizuka, S. et al. (2011) Mol. Cell Biol. 31:783.
Lu, X. et al. (2013) Bone 54:157.
Zhang, Y. et al. (2011) Eur. J. Oral. Sci. 119:Suppl 1:280.
Sonoda, A. et al. (2009) J. Biol. Chem. 284:27176.
Entrez Gene IDs
258 (Human); 11698 (Mouse)
AI1F, AMBN, Ameloblastin
Citations for Recombinant Mouse Ameloblastin Protein, CF
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Product Documents for Recombinant Mouse Ameloblastin Protein, CF
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