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Recombinant Mouse 2B4/CD244/SLAMF4 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 3514-2B

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3514-2B-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse 2B4/CD244/SLAMF4 protein
Mouse 2B4
(His17-Asn221)
Accession #Q07763
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His17

Predicted Molecular Mass

50 kDa (monomer)

SDS-PAGE

80 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rm2B4/Fc Chimera at 5 µg/mL (100 µL/well) can bind rmCD48 with a linear range of 0.15-10 μg/mL.

Formulation, Preparation and Storage

3514-2B
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 2B4/CD244/SLAMF4

2B4, also known as CD244 and SLAMF4, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig‑like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact in a homophilic manner (1‑4). The mouse 2B4 cDNA encodes a 397 amino acid (aa) precursor that includes a 19 aa signal sequence, a 207 aa ECD with one Ig-like V-type and one C2-type Ig-like domain, a 21 aa transmembrane segment, and a 150 aa cytoplasmic domain with four ITSMs (5, 6). A short splice variant of mouse 2B4 contains only one ITSM (6). Within the ECD, mouse 2B4 shares 46% and 68% aa sequence identity with human and rat 2B4, respectively. The ECD of mouse 2B4 shares 17‑26% aa sequence identity with comparable regions of mouse BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. 2B4 is expressed on all NK cells, gamma delta T cells, monocytes, some CD4+ and CD8+ T cells, and dendritic cells (7). CD48 mediates 2B4+ cell interactions with nearly all hematopoietic cell types, including cells of the same type (8‑10). 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation (7‑13). The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors (12, 13). Ligation of 2B4 with antibodies or CD48 constructs can directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation (9, 12). 2B4 can also induce signaling through CD48 (10, 14). The inhibitory effect is associated with the long form of 2B4, while activation is associated with the short form (9, 15).

References

  1. Bhat, R. et al. (2006) J. Leukocyte Biol. 79:417.
  2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
  3. McNerney, M.E. et al. (2005) Mol. Immunol. 42:489.
  4. Assarsson, E. et al. (2005) J. Immunol. 175:2045.
  5. Mathew, P.A. et al. (1993) J. Immunol. 151:5328.
  6. Stepp, S.E. et al. (1999) Eur. J. Immunol. 29:2392.
  7. Nakajima, H. et al. (1999) Eur. J. Immunol. 29:1676.
  8. Lee, K.M. et al. (2006) Blood 107:3181.
  9. Mooney, J.M. et al. (2004) J. Immunol. 173:3953.
  10. Assarsson, E. et al. (2004) J. Immunol. 173:174.
  11. Bryceson, Y.T. et al. (2006) Blood 107:159.
  12. Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.
  13. McNerney, M.E. et al. (2005) Blood 106:1337.
  14. Messmer, B. et al. (2006) J. Immunol. 176:4646.
  15. Schatzle, J.D. et al. (1999) Proc. Natl. Acad. Sci. 96:3870.

Alternate Names

CD244, NAIL, NKR2B4, Nmrk, SLAMF4

Entrez Gene IDs

51744 (Human); 18106 (Mouse); 685808 (Rat); 102132700 (Cynomolgus Monkey)

Gene Symbol

CD244

UniProt

Additional 2B4/CD244/SLAMF4 Products

Product Documents for Recombinant Mouse 2B4/CD244/SLAMF4 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse 2B4/CD244/SLAMF4 Fc Chimera Protein, CF

For research use only

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