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Recombinant Monkeypox Virus H3L His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 11277-MX

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11277-MX-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 H3L protein
Met1-Pro278, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1 & Lys5

Predicted Molecular Mass

33 kDa

SDS-PAGE

36-40 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Monkeypox Virus H3L His-tag Protein, CF

Recombinant Monkeypox Virus H3L His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Monkeypox Virus H3L His-tag Protein (Catalog # 11277-MX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36-40 kDa.

Formulation, Preparation and Storage

11277-MX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: H3L

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ~14 kDa and H3L ~32kDa), an envelope glycoprotein, A35R ~15 kDa), a receptor glycoprotein that mimics IFN-alpha/beta (B16, ~37 kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14 kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2, 3). The H3L subunit is an envelope protein that assists in virion attachment (4). It belongs to the chordopoxvirinae protein H3 family and is 278 ammino acids long (2). H3L has been looked at for use in testing kits and immunoassays due to its high abundance in MPXV patients after viral infection (5). Other studies have shown that the H3L subunit plays a role in the Vaccinia virus based vaccine's ability to cross vaccinate from MPXV (6).

References

  1. Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
  2. Farahat, R.A. et al. (2022) Infez Med. 30:372.
  3. Schelkunov, S.N. et al. (2002) Virology 297:172.
  4. Nicolas, L. et al. (2022) Travel. Med. Infect. Dis. 49:102402.
  5. Yefet, R. et al. (2022) medRxiv doi: https://doi.org/10.1101/2022.08.22.22278946.
  6. Ahmed, S.F. et al. (2022) Viruses 14:1960.

Long Name

Envelope Protein H3

Entrez Gene IDs

928895 (Monkeypox Virus Zaire-96-I-16)

Gene Symbol

OPG108

UniProt

Additional H3L Products

Product Documents for Recombinant Monkeypox Virus H3L His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Monkeypox Virus H3L His-tag Protein, CF

For research use only

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