Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Monkeypox Virus A35R His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 11285-MX

Catalog #
Availability
Size / Price
Qty
Loading...
11285-MX-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 A35R protein
Arg58-Thr181, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg58

Predicted Molecular Mass

14 kDa

SDS-PAGE

13-21 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

11285-MX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A35R

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ,~14 kDa and H3L, ~32kDa), an envelope glycoprotein (A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2-3). The envelope glycoprotein A35R is highly conserved among poxviruses. It is not required for replication, but it was reported  to play a role in virulence (4). In addition, A35R was showed to inhibit MHC class II antigen presentation (5).

References

  1. Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
  2. Farahat, R.A. et al. (2022) Infez Med. 30:372.
  3. Schelkunov, S.N. et al. (2002) Virology 297:172.
  4. Roper, R. L. (2006) J. Virol. 80:306.
  5. Rehm, K. E. et al. (2010) Virology 397:176.

Long Name

EEV Envelope Glycoprotein

Entrez Gene IDs

928958 (Monkeypox Virus Zaire-96-I-16)

Gene Symbol

OPG161

UniProt

Additional A35R Products

Product Documents for Recombinant Monkeypox Virus A35R His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Monkeypox Virus A35R His-tag Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=