Recombinant Monkeypox Virus A30L His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11282-MX

Analyzed by SEC-MALS
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11282-MX-100
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Monkeypox Virus A30L His-tag Protein (11282-MX)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession #

NP_536567.1

Conjugate

Unconjugated

Applications

Bioactivity

View all A30L Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 A30L protein
Gln22-Leu146, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln22 inferred from enzymatic pyroglutamate treatment revealing Ser23

Predicted Molecular Mass

15 kDa

SDS-PAGE

22-26 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Monkeypox Virus A30L His-tag Protein, CF

Recombinant Monkeypox Virus Zaire-96-I-16 A30L His-tag Protein SEC-MALS.

Recombinant Monkeypox Virus Zaire-96-I-16 A30L His-tag Protein (Catalog # 11282-MX) has a molecular weight (MW) of 20.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.

Recombinant Monkeypox Virus A30L His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Monkeypox Virus A30L His-tag Protein (Catalog # 11282-MX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 22-26 kDa.

Formulation, Preparation and Storage

11282-MX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A30L

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ~14 kDa and H3L ~32 kDa), an envelope glycoprotein, A35R ~15 kDa), a receptor glycoprotein that mimics IFN-alpha/beta (B16, ~37 kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14 kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36 kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2, 3). The A30L subunit is 146 amino acids long and one of the two envelope proteins responsible for allowing for viral entry into a host cell and starting cell to cell fusion (4). The A30L subunit has been the focus of multiple studies for vaccination purposes for not only monkeypox, but for smallpox as well (4). The A30L subunit has also been looked into for development in detection kits for MPXV (5).

References

  1. Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
  2. Farahat, R.A. et al. (2022) Infez Med. 30:372.
  3. Schelkunov, S.N. et al. (2002) Virology 297:172.
  4. Rinat, A.M. et al. (2013) Journal of Vaccines http://dx.doi.org/10.1155/2013/618324.
  5. Dumont, C. et al. (2014) PLOS One 9:e96930.

Long Name

Envelope Protein A28 Homolog

Entrez Gene IDs

928965 (Monkeypox Virus Zaire-96-I-16)

Gene Symbol

OPG155

UniProt

NP_536567.1

Additional A30L Products

Product Documents for Recombinant Monkeypox Virus A30L His-tag Protein, CF

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Product Specific Notices for Recombinant Monkeypox Virus A30L His-tag Protein, CF

For research use only

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