Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived mers-cov Nucleocapsid protein Met1-Thr411, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Sequence has been confirmed by mass spectrometry
Predicted Molecular Mass	46 kDa
SDS-PAGE	49-55 kDa, under reducing conditions
Activity	Bioassay data are not available.

Recombinant MERS-CoV Nucleocapsid His-tag Protein, CF Scientific Data Examples

	Recombinant MERS-CoV Nucleocapsid His-tag Protein SDS-PAGE 2 μg/lane of Recombinant MERS-CoV Nucleocapsid His-tag Protein (10521-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 49-55 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10521-CV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nucleocapsid

MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelope, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (1, 2). The MERS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains an Intrinsically Disordered Region (3) and an RNA binding domain (4), the linker region interacts with the M protein and the C-terminal region contains a self-association domain (1, 2). The MERS-CoV N protein shares 46.3% and 4.5% amino acid sequence identity with SARS-CoV-1 and SARS-CoV-2 N protein, respectively. MERS-CoV N proteins have been shown to inhibit Type I Interferon(IFN) production(1). In addition, the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity, making it a promising therapeutic target (5-7).

Item	Value
References	Li, Y. et al. (2019) Engineering. 5:940. Hurst, K. R. et al. (2009) J. Virol. 83:7221. Wang, Y. et al. (2015) Acta. Crystallogr. F. Struct. Biol. Commun. 71:977. Papageorgiou, N. et al. (2016) Acta. Crystallogr. D. Struct. Biol. 72:192. Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629. Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287. Chang, C-K. et al.(2016) Drug Discov. Today. 21:562.
Long Name	Nucleocapsid Protein
Entrez Gene IDs	918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)
Alternate Names	N Protein, Nucleocapsid

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Recombinant MERS-CoV Nucleocapsid His-tag Protein, CF

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