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Recombinant MERS-CoV Nucleocapsid His-tag Protein, CF

Catalog # 10521-CV | R&D Systems, Inc. a Bio-Techne Brand
Sf21 Insect Cell Expressed, Over 95% Purity
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10521-CV-100

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Conjugate

Unconjugated

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mers-cov Nucleocapsid protein
Met1-Thr411, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Sequence has been confirmed by mass spectrometry

Predicted Molecular Mass

46 kDa

SDS-PAGE

49-55 kDa, under reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant MERS-CoV Nucleocapsid His-tag Protein, CF

Recombinant MERS-CoV Nucleocapsid His-tag Protein SDS-PAGE

Recombinant MERS-CoV Nucleocapsid His-tag Protein SDS-PAGE

2 μg/lane of Recombinant MERS-CoV Nucleocapsid His-tag Protein (10521-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 49-55 kDa.

Formulation, Preparation and Storage

10521-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nucleocapsid

MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelope, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (1, 2). The MERS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains an Intrinsically Disordered Region (3) and an RNA binding domain (4), the linker region interacts with the M protein and the C-terminal region contains a self-association domain (1, 2). The MERS-CoV N protein shares 46.3% and 4.5% amino acid sequence identity with SARS-CoV-1 and SARS-CoV-2 N protein, respectively. MERS-CoV N proteins have been shown to inhibit Type I Interferon(IFN) production(1). In addition, the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity, making it a promising therapeutic target (5-7).

References

  1. Li, Y. et al. (2019) Engineering. 5:940.
  2. Hurst, K. R. et al. (2009) J. Virol. 83:7221.
  3. Wang, Y. et al. (2015) Acta. Crystallogr. F. Struct. Biol. Commun. 71:977.
  4. Papageorgiou, N. et al. (2016) Acta. Crystallogr. D. Struct. Biol. 72:192.
  5. Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
  6. Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.
  7. Chang, C-K. et al.(2016) Drug Discov. Today. 21:562.

Long Name

Nucleocapsid Protein

Alternate Names

N Protein

Entrez Gene IDs

918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)

Gene Symbol

N

UniProt

Product Documents for Recombinant MERS-CoV Nucleocapsid His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant MERS-CoV Nucleocapsid His-tag Protein, CF

For research use only

Reconstitution Calculator

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