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Recombinant Human Wnt-16b Protein

Catalog # 7790-WN | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
7790-WN-025/CF

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With Carrier
7790-WN-025

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Wnt-16b protein
Asn30-Lys365

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn30

Predicted Molecular Mass

37.6 kDa

SDS-PAGE

45-65 kDa, reducing conditions

Activity

Measured in a cell proliferation/survival assay using 3T3‑L1 mouse embryonic fibroblast adipose-like cells.
The ED50 for this effect is 0.4‑1.6 μg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7790-WN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7790-WN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-16b

Wnt‑16 is a 40 kDa protein within the Wnt family of secreted, highly conserved, cysteine‑rich, palmitoylated cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Wnt‑16a and Wnt‑16b isoforms in humans differ in the signal sequence and the first two amino acids (aa) of the mature protein (2, 3). Wnt‑16b is the more conserved isoform and is widely expressed, while Wnt‑16a is expressed mainly in the human pancreas (3). Mature human Wnt‑16b shares 92%, 93%, and 95% aa sequence identity with mouse/rat, rabbit/porcine/equine, and bovine Wnt‑16, respectively. Wnt‑16 expression is detected on uterine stroma adjacent to the luminal epithelium during implantation (4). It is up‑regulated during the first embryonic lymphoid progenitor differentiation (5). Congenital heart defects correlate with elevated Wnt‑16 in mouse embryos and human amniotic fluid (6). Low cortical bone thickness and bone mineral density correlate with deletion of Wnt‑16 in mice and a Wnt‑16 missense SNP in humans (7). Wnt‑16 is over‑expressed in cells undergoing replicative senescence, and is up‑regulated in articular cartilage by injury and osteoarthritis (8, 9). Wnt‑16b expression in skin is up‑regulated in human basal cell carcinomas, enhancing cell survival (10). Its expression is also up‑regulated by DNA damage (radiation and chemotherapy) in stroma surrounding prostate tumors, causing enhanced survival and treatment resistance in the tumor cells (11). Pre‑B acute lymphoblastic leukemia with t(1;19) translocation, creating an E2A‑Pbx1 fusion protein, also causes up‑regulation of Wnt‑16 that confers resistance to apoptosis (12, 13). Wnt‑16 signaling through both canonical and JNK‑mediated (non‑canonical) pathways is reported (8‑10).

References

  1. Clevers, H. and R. Nusse (2012) Cell 149:1192.
  2. Katoh, H. and M. Katoh 2005) Oncol. Rep. 13:771.
  3. Fear, M.W. et al. (2000) Biochem. Biophys. Res. Commun. 278:814.
  4. Hayashi, K. et al. (2009) Biol. Reprod. 80:989.
  5. Corrigan, P.M. et al. (2009) Stem Cells Dev. 18:759.
  6. Nath, A.K. et al. (2009) PLoS ONE 4:e4221.
  7. Zheng, H.F. et al. (2012) PLoS Genet. 8:31002745.
  8. Dell’accio, F. et al. (2008) Arthritis Rheum. 58:1410.
  9. Binet, R. et al. (2009) Cancer Res. 69:9183.
  10. Teh, M.T. et al. (2006) J. Cell Sci. 120:330.
  11. Sun, Y. et al. (2012) Nat. Med. 18:1359.
  12. McWhirter, J.R. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11464.
  13. Mazieres, J. et al. (2005) Oncogene 24:5396.

Long Name

Wingless-type MMTV Integration Site Family, Member 16B

Alternate Names

WNT16, Wnt16b

Entrez Gene IDs

51384 (Human); 93735 (Mouse); 500047 (Rat)

Gene Symbol

WNT16

UniProt

Product Documents for Recombinant Human Wnt-16b Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Wnt-16b Protein

For research use only

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