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Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF

Catalog # 11160-WN | R&D Systems, Inc. a Bio-Techne Brand
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11160-WN-010

Key Product Details

Source

CHO

Structure / Form

Non-covalent complex

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Wnt-1 protein
Human Wnt-1
(Ala28-Leu370)
Accession # P04628.1
Human sFRP-1
(Ser32-Lys314)
Accession # AAB70793.1
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala28 (Wnt-1) & Ser32 (sFRP-1)

Predicted Molecular Mass

38 kDa (Wnt-1) & 33 kDa (sFRP-1)

SDS-PAGE

34-58 kDa, under reducing conditions.

Activity

Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells.
The ED50 for this effect is 25.0-250 ng/mL.

Scientific Data Images for Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF

Recombinant Human Wnt-1/sFRP-1 Complex Bioactivity.

Recombinant Human Wnt-1/sFRP-1 Complex (Catalog # 11160-WN) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 25.0-250 ng/mL.

Formulation, Preparation and Storage

11160-WN
Formulation Supplied as a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Wnt-1

Wnt-1 is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins that are important for normal developmental processes (1). Human Wnt-1 cDNA encodes a 370 amino acid (aa) protein that contains four potential N-linked glycosylation sites plus 23 cysteines (2, 3). When secreted, it exists as a 343 aa glycoprotein with a molecular weight of 42 to 48 kDa. Human Wnt -1 shares a 99.7% aa sequence identity with mouse Wnt-1. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta-catenin levels (4). As intracellular beta-catenin levels rise, beta-catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (5). Wnt-1 is known to bind to sFRPs, a family of secreted molecules that contain an N-terminal cysteine-rich domain (CRD) highly similar to the CRDs of the Frizzled family receptors (6,7). More than one molecule of sFRP is suggested to participate in the interaction with each Wnt molecule (7). R&D Systems produces biologically active human Wnt-1 protein in a complex with human sFRP-1, and this Wnt-1/sFRP-1 complex is stable in the physiological buffer without detergent.

References

  1. Willert, K. and R. Nusse (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. Van Ooyen, A. et al. (1985) EMBO J. 4:2905.
  3. Burrus, L.W. and A.P. McMahon (1995) Exp. Cell Res. 220:363.
  4. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  5. Korinek, V. et al. (1997) Science 275:1784.
  6. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  7. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.

Long Name

Wingless-type MMTV Integration Site Family, Member 1

Alternate Names

Int-1, Wnt1

Entrez Gene IDs

7471 (Human); 22408 (Mouse)

Gene Symbol

WNT1

Product Documents for Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Wnt-1/sFRP-1 Complex Protein, CF

For research use only

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