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Recombinant Human VSTM2B His-tag Protein, CF

Catalog # 10061-VT | R&D Systems, Inc. a Bio-Techne Brand
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10061-VT-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human VSTM2B protein
Ala29-Thr263, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala29

Predicted Molecular Mass

26 kDa

SDS-PAGE

37-45 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 1-6 μg/mL.

Scientific Data Images for Recombinant Human VSTM2B His-tag Protein, CF

Recombinant Human VSTM2B His-tag Protein Bioactivity

Recombinant Human VSTM2B His-tag Protein Bioactivity

Recombinant Human VSTM2B (Catalog # 10061-VT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-6 μg/mL.
Recombinant Human VSTM2B His-tag Protein SDS-PAGE

Recombinant Human VSTM2B His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human VSTM2B was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37 - 45 kDa.

Formulation, Preparation and Storage

10061-VT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: VSTM2B

VSTM2B, or V-set and transmembrane domain-containing protein 2B is a membrane protein which includes a 235 amino acid (aa) extracellular domain that contains an Ig-like V-type domain, a 21 aa transmembrane domain, and one aa in cytoplasm. This extracellular domain shares 45% aa identity human VSTM2A. The mature extracellular domain of human VSTM2B shares 86% and 85% aa sequence identity for the mouse and rat homolog, respectively. Currently, both the expression pattern and biological functions of VSTM2B remain unknown. our in house data show that VSTM2B inhibits the human T cell activation, including anti-CD3 induced IL-2, IFN-gamma secretion, and T cell proliferation.

Long Name

V-Set And Transmembrane Domain Containing 2B

Alternate Names

V-set and transmembrane domain-containing protein 2B

Entrez Gene IDs

342865 (Human); 58188 (Mouse); 361560 (Rat)

Gene Symbol

VSTM2B

UniProt

Product Documents for Recombinant Human VSTM2B His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human VSTM2B His-tag Protein, CF

For research use only

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