Recombinant Human Vitronectin Protein, Animal-Free
R&D Systems, part of Bio-Techne | Catalog # BT-VTN-AFL
N-terminal Variant
Key Product Details
Product Specifications
Source
E. coli-derived human Vitronectin protein
Val62-Glu292 with a C-terminal 6-His tag
Produced using non-animal reagents in an animal-free laboratory.
Val62-Glu292 with a C-terminal 6-His tag
Produced using non-animal reagents in an animal-free laboratory.
Purity
>90%, by HPLC.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met & Val62
Predicted Molecular Mass
27 kDa
Activity
Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells.
The ED50 for this effect is 10.0-120 ng/mL.
The ED50 for this effect is 10.0-120 ng/mL.
Scientific Data Images for Recombinant Human Vitronectin Protein, Animal-Free
Animal-Free™ Recombinant Human Vitronectin Protein Bioactivity.
Animal-Free™ Recombinant Human Vitronectin (Catalog # BT-VTN-AFL) supports the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 10.0-120 ng/mL.Formulation, Preparation and Storage
BT-VTN-AFL
| Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and TCEP with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in sterile water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Vitronectin
A truncated, E. coli derived version of
human Vitronectin (aa62-292) has been optimized for large scale stem cell
culture applications. This Vitronectin contains the RGD sequence necessary for
interaction with cell-surface receptors to maintain its ability to act as a
stem cell substrate.
References
- Lu, X. et al. (2024) Cells 13:1566.
Alternate Names
Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN
Gene Symbol
VTN
UniProt
Additional Vitronectin Products
Product Documents for Recombinant Human Vitronectin Protein, Animal-Free
Manufacturing Specifications
Animal-Free Manufacturing ConditionsOur dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.
Production
- All molecular biology procedures use animal-free media and dedicated labware.
- Dedicated fermentors are utilized in committed animal-free areas.
Purification
- Protein purification columns are animal-free.
- Bulk proteins are filtered using animal-free filters.
- Purified proteins are stored in animal-free containers in a dedicated cold storage room.
- Low Endotoxin Level.
- No impairment of biological activity.
- High quality product obtained under stringent conditions.
- For ex vivo research or bioproduction, additional documentation can be provided.
Product Specific Notices for Recombinant Human Vitronectin Protein, Animal-Free
For research use only
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