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Recombinant Human VHR Protein, CF

Catalog # 1654-VH | R&D Systems, Inc. a Bio-Techne Brand
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1654-VH-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived human VHR protein
Ser2-Pro185, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

21 kDa

SDS-PAGE

23 kDa, reducing conditions

Activity

Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >100 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

1654-VH
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: VHR

Vaccinia Virus VH1-related Phosphatase (VHR), also known as Dual-Specificity Phosphatase 3 (DUSP3), removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). It belongs to a family of phosphatases that selectively dephosphorylate MAP kinases. VHR has been shown to dephosphorylate cellular ERK1, ERK2 and JNK, but not p38 (2, 3). Phosphorylation of VHR at tyrosine 138 by ZAP-70 enhances inhibition of ERK2, suggesting a role for VHR in modulating T-cell receptor responses (3). The enzymatic mechanism for VHR has been studied in detail (4) and its X-ray crystal structure has been characterized (5). Its well-defined biochemistry has made it useful in screening assays for compounds that inhibit phosphatases (6).

References

  1. Ishibashi, T. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12170.
  2. Todd, J.L. et al. (1999) J. Biol. Chem. 274:13271.
  3. Alonso, A. et al. (2003) Nat. Immunol. 4:44.
  4. Zhang, Z.-Y. et al. (1995) Biochemistry 34:16088.
  5. Yuvaniyama, J. et al. (1996) Science 272:1328.
  6. Ueda, K. et al. (2002) FEBS Lett. 525:48.

Long Name

Vaccinia Virus VH1-related Phosphatase

Alternate Names

DUSP3

Entrez Gene IDs

1845 (Human)

Gene Symbol

DUSP3

UniProt

Additional VHR Products

Product Documents for Recombinant Human VHR Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human VHR Protein, CF

For research use only

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Setting the standard in quality research reagents