Product Specifications for Recombinant Human VG5Q Protein, CF
Mouse myeloma cell line, NS0-derived human VG5Q protein Ala2-Glu714, with an N-terminal 9-His tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
120-140 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. Immobilized rhVG5Q at 1 µg/mL (100 µL/well) can bind rhTWEAK with a linear range of 0.8-50 ng/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
VG5Q, also known as AGGF1, was identified by its association with Klippel-Trenaunay syndrome (KTS), a congenital vascular morphogenesis disorder (1 - 3). Two genetic alterations found in KTS lead to aberrant expression or function of VG5Q: a chromosomal translocation that falls within the VG5Q promoter and a glutamate to lysine point mutation at position 133 (1). The human VG5Q cDNA encodes a 714 amino acid (aa) precursor with a coiled coil domain, an FHA domain, a G-patch domain, and an OCRE domain. FHA domains bind phosphorylated threonine and tyrosine residues (4, 5). G-patch and OCRE motif-containing proteins associate with RNA and are implicated in RNA metabolism (6, 7). Two splice variants (109 aa and 176 aa) of human VG5Q have C-terminal deletions and contain alternate sequences at their C-termini. The FHA, G-patch, and OCRE domains are absent in both of these splice variants. Human VG5Q shares 78% and 89% aa identity with mouse and canine VG5Q, respectively. Bovine VG5Q has a C-terminal truncation following position 294 but shares 85% aa identity with the corresponding region of human VG5Q. VG5Q is expressed by vascular endothelial cells in many tissues (1). It appears to be secreted and promotes endothelial cell proliferation following interactions with endothelial cell surfaces (1). VG5Q also directly interacts with TWEAK (1), a TNF superfamily ligand with angiogenic properties (8). The FHA, G-patch, and OCRE domains of VG5Q suggest additional intracellular functions.
Tian, X-L. et al. (2004) Nature 427:640.
Timur, A.A et al. (2005) Cell. Mol. Life Sci. 62:1434.
Lambrechts, D. and P. Carmeliet (2004) Nature 427:592.
Durocher, D. and S.P. Jackson (2002) FEBS Lett. 513:58.
Wang, P. et al. (2000) J. Mol. Biol. 302:927.
Aravind, L. and E.V. Koonin (1999) Trends Biochem. Sci. 24:342.
Callebaut, I. and J.P. Mornon (2005) Bioinformatics 21:699.
Wiley, S.R. and J.A. Winkles (2003) Cytokine Growth Factor Rev. 14:241.
Vasculogenesis Gene on 5q/Angiogenic Factor with G Patch and FHA Domains 1
Entrez Gene IDs
AGGF1, Angiogenic factor VG5Q, FLJ10283hVG5Q, GPATC7G patch domain-containing protein 7, GPATCH7HUS84971, HSU84971, VG5Q, VG5QVasculogenesis gene on 5q protein, angiogenic factor with G patch and FHA domains 1
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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