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Recombinant Human VG5Q Protein, CF

Bio-Techne includes R&D Systems | Catalog # 3048-VG

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3048-VG-025

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human VG5Q protein
Ala2-Glu714, with an N-terminal 9-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

82.0 kDa

SDS-PAGE

120-140 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rhVG5Q at 1 µg/mL (100 µL/well) can bind rhTWEAK with a linear range of 0.8-50 ng/mL.

Formulation, Preparation and Storage

3048-VG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VG5Q

VG5Q, also known as AGGF1, was identified by its association with Klippel-Trenaunay syndrome (KTS), a congenital vascular morphogenesis disorder (1 - 3). Two genetic alterations found in KTS lead to aberrant expression or function of VG5Q: a chromosomal translocation that falls within the VG5Q promoter and a glutamate to lysine point mutation at position 133 (1). The human VG5Q cDNA encodes a 714 amino acid (aa) precursor with a coiled coil domain, an FHA domain, a G-patch domain, and an OCRE domain. FHA domains bind phosphorylated threonine and tyrosine residues (4, 5). G-patch and OCRE motif-containing proteins associate with RNA and are implicated in RNA metabolism (6, 7). Two splice variants (109 aa and 176 aa) of human VG5Q have C-terminal deletions and contain alternate sequences at their C-termini. The FHA, G-patch, and OCRE domains are absent in both of these splice variants. Human VG5Q shares 78% and 89% aa identity with mouse and canine VG5Q, respectively. Bovine VG5Q has a C-terminal truncation following position 294 but shares 85% aa identity with the corresponding region of human VG5Q. VG5Q is expressed by vascular endothelial cells in many tissues (1). It appears to be secreted and promotes endothelial cell proliferation following interactions with endothelial cell surfaces (1). VG5Q also directly interacts with TWEAK (1), a TNF superfamily ligand with angiogenic properties (8). The FHA, G-patch, and OCRE domains of VG5Q suggest additional intracellular functions.

References

  1. Tian, X-L. et al. (2004) Nature 427:640.
  2. Timur, A.A et al. (2005) Cell. Mol. Life Sci. 62:1434.
  3. Lambrechts, D. and P. Carmeliet (2004) Nature 427:592.
  4. Durocher, D. and S.P. Jackson (2002) FEBS Lett. 513:58.
  5. Wang, P. et al. (2000) J. Mol. Biol. 302:927.
  6. Aravind, L. and E.V. Koonin (1999) Trends Biochem. Sci. 24:342.
  7. Callebaut, I. and J.P. Mornon (2005) Bioinformatics 21:699.
  8. Wiley, S.R. and J.A. Winkles (2003) Cytokine Growth Factor Rev. 14:241.

Long Name

Vasculogenesis Gene on 5q/Angiogenic Factor with G Patch and FHA Domains 1

Alternate Names

AGGF1

Entrez Gene IDs

55109 (Human)

Gene Symbol

AGGF1

UniProt

Additional VG5Q Products

Product Documents for Recombinant Human VG5Q Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human VG5Q Protein, CF

For research use only

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Reconstitution Calculator

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