Product Specifications for Recombinant Human Versican Isoform V3 His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human Versican protein Leu21-Tyr646, with a C-terminal 6-His tag
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
78-89 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Human Versican Isoform V3 (Catalog # 3054-VN) is coated at 0.25 μg/mL, 100 μL/well, Biotinylated Hyalurinan binds with an ED50 of 0.25-1.5 ng/mL.
Scientific Data Examples for Recombinant Human Versican Isoform V3 His-tag Protein, CF
Recombinant Human Versican Isoform V3 His-tag Protein Binding Activity
When Recombinant Human Versican Isoform V3 (Catalog # 3054-VN) is coated at 0.25 μg/mL, 100 μL/well, Biotinylated Hyalurinan binds with an ED50 of 0.25-1.5 ng/mL.
Recombinant Human Versican Isoform V3 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human Versican Isoform V3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-89 kDa and 67-80 kDa, respectively.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, ≤ -20 °C under sterile conditions after reconstitution.
Versican (versatile proteoglycan; also known as PG-M, CSPG2, ERVR, GHAP, WGN, WGN1) is a 500-600 kDa, secreted member of the large aggregating chondroitin sulfate proteoglycan family, which includes aggrecan, neurocan, and brevican (1-3). The canonical sequence of human Versican (Isoform V0) is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region, two large, alternately spliced GAG attachment domains (GAG alpha and GAG beta), two EGF-like domains, a selectin-like region, and a complement regulatory protein-like domain (2). Human Versican has multiple alternative splice variants that include variations mainly in the GAG regions. This product is the Isoform V3 which includes one mutation at aa 348 (Pro to Arg) and lacks of the entire GAG domains (aa 349-3089) compared to Isoform V0. Versican is known to associate with hyaluronan (4), tenascin (5-7), fibronectin (8), fibulin (9), and CD44 (10) in the extracellular matrix that may contribute to the malignant properties of tumor cells (11). It has an anti-adhesive effect on cells, facilitating tumor migration and invasion (12). The multiple alternative splice variants of human Versican could affect cell-cell and cell-matrix adhesion (13-15), migration (16), proliferation, apoptosis (17), and mesenchymal-epithelial transition (18). The Versican gene is expressed in several different tissues, and it is over-expressed in several cancer types, including prostate (19), brain (20), ovary (21), breast (22) and melanoma (11).
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