Product Specifications for Recombinant Human VEGFR2/KDR His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human VEGFR2/KDR/Flk-1 protein Ala20-Glu764, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 0.350-3.50 μg/mL.
Scientific Data Examples for Recombinant Human VEGFR2/KDR His-tag Protein, CF
Recombinant Human VEGFR2/KDR/Flk-1 His-tag Protein Bioactivity.
Recombinant Human VEGFR2/KDR/Flk-1 His-tag Protein (Catalog # 10964-KD) inhibits the Recombinant Human VEGF165 (293-VE) dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.350-3.50 μg/mL.
Recombinant Human VEGFR2/KDR His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human VEGFR2/KDR His-tag Protein (Catalog # 10964-KD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 114-126 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 1.00 mg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Vascular endothelial Growth Factor Receptor 2 (VEGFR2), also known as FLK-1, KDR, and CD309, is a type I single-pass membrane receptor. Mature VEGFR2 contains a 745 amino acid extracelluar domain with seven immunoglobulin-like repeats, 21 transmembrane domain and 571 cytoplasmic domain. Within the extracellular domain, human VEGFR2 shares 80% homology with that of mouse and rat. VEGFR2, VEGFR1(Flt-1) and VEGFR3(Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors have almost exclusive expression in the endothelial cells and play essential roles in vasculogenesis and angiogenesis. VEGFR2 is the receptor for VEGF-A, VEGF-C, VEGF-D, and VEGF-E (viral homologs) (1, 2). Monomeric VEGFR2 dimerizes after binding to dimeric ligand and phosphorylate the Tyr in the cytoplasmic domain (3). VEGFR2 can also form heterodimer with VEGFR1 and VEGFR3 (4-6). Alternative splicing isoforms 2 and 3 which lack the transmembrane and cytoplasmic domains function as decoy receptors (7, 8). Targeting the signaling pathways of VEGFR1 and VEGFR2 are potential therapeutic targets for the treatment of inflammation and multiple tumors including breast, gastric, and lung carcinomas (9-12). Cancer immunotherapies using VEGF and VEGFR2 monoclonal antibodies may also be effective in combination with programmed cell death protein 1 (PD-1)/ programmed cell death ligand 1 (PD-L1) immune checkpoint blockade (13).
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Wise, L.M. et al. (2012) Cell Microbiol. 13:1376.
Lepanen, V.M. et al. (2010) Proc. Natl. Acad. Sci. USA 107:2425.
Cai, M. et al. (2017) Vascul. Pharmacol. 88:11.
Nilsson, I. et al. (2010) EMBO J. 29:1377.
Dixelius, J. et al. (2003) J. Biol. Chem. 278:40973.
Albuquerque, R.J. et al. (2009) Nat. Med. 15:1023.
Jin, P. et al. (2008) Arthritis Res. Ther. 10:R73.
Shibuya, M. (2015) Endocr Metab Immune Disord Drug Targets. 15:135.
Farzaneh Behelgardi, M. et al. (2020), Mol Biol Rep. 47:2061.
Lian, L. et al. (2019) BMC Cancer. 19:183.
Hu, C. et al. (2019) Onco. Targets Ther. 12:933.
Gao, F. et al. (2020) Curr. Cancer Drug Targets. 20:3.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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