Recombinant Human VEGF 189 (aa 27-215) Protein

Name: Recombinant Human VEGF 189 (aa 27-215) Protein
Brand: R&D Systems
SKU: 8147-VE
Price: 388 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8147-VE

Citations (2)

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Key Product Details

Source:	CHO
Accession #:	AAA36804
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human VEGF 189 (aa 27-215) Protein

Product Specifications for Recombinant Human VEGF 189 (aa 27-215) Protein

Source	Chinese Hamster Ovary cell line, CHO-derived human VEGF protein Ala27-Arg215
Purity	>95%, by SDS-PAGE with silver staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala27
Predicted Molecular Mass	22 kDa (monomer)
SDS-PAGE	22-30 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 4-40 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

8147-VE

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8147-VE/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

VEGF₁₈₉ (Vascular Endothelial Growth Factor-A of 189 amino acids) is a 50-52 kDa homodimeric glycoprotein member of the PDGF/VEGF family of molecules (1, 2). A large number of human VEGF isoforms are generated by alternative splicing, including VEGF₁₈₉ which lacks amino acids (aa) 166-182 of the full length protein (VEGF₂₀₆) (3-5). Mature human VEGF₁₈₉ shares 88% aa sequence identity with mouse VEGF₁₈₈. VEGF₁₈₉ binds to heparan sulfate both on the cell surface and in the extracellular matrix (ECM) where it retains bioactivity (6, 7). It can undergo proteolytic cleavage from the ECM (at Arg136-Ala137), creating a soluble fragment with increased bioactivity (6, 7). VEGF₁₈₉ is widely expressed including by vascular smooth muscle cells (3), chondrocytes (8), fibroblasts (9), neuronal and glial cells (10), and neutrophils (11). VEGF₁₈₉ interacts with various receptors including VEGF R1, VEGF R2, Neuropilin-1, and Integrins aVb3, aVb5, a3b1, and a5b1 (6, 12-14). VEGF₁₈₉ exerts complex effects in angiogenesis. It is proapoptotic towards quiescent vascular endothelium but is proangiogenic towards endothelium that has committed to the angiogenic process (6, 14). In particular, venous endothelial cells are highly responsive towards VEGF₁₈₉, while the responsiveness of arterial endothelium is primarily restricted to the regulation of migration (7). In addition to its vascular effects, immobilized VEGF₁₈₉ serves as an anchor for cells expressing Integrin avb3, and it acts as both a chemotactic and chemokinetic factor for migrating neutrophils (11, 13, 14).

Item	Value
References	Woolard, J. et al. (2009) Microcirculation 16:572. Ferrara, N. et al. (2003) Nat. Med. 9:669. Tischer, E. et al. (1991) J. Biol. Chem. 266:11947. Leung, D.W. et al. (1989) Science 246:1306. Keck, P.J. et al. (1989) Science 246:1309. Plouet, J. et al. (1997) J. Biol. Chem. 272:13390. Herve, M-A. et al. (2005) Exp. Cell Res. 309:24. Cramer, T. et al. (2004) Osteoarthritis Cartilage 12:433. Detmar, M. et al. (1997) J. Invest. Dermatol. 108:263. Tang, H. et al. (2013) Neurobiol. Aging 34:1412. Houck, K.A. et al. (1992) J. Biol. Chem. 267:26031. Herve, M-A. et al. (2008) Am. J. Pathol. 172:167. Hutchings, H. et al. (2003) FASEB J. 17:1520. Vintonenko, N. et al. (2011) Cell Adh. Migr. 5:332.
Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names	MVCD1, VAS, VEGF, VEGF-A, VEGFA, VEGFMGC70609, VPF, VPFvascular endothelial growth factor, Vascular permeability factor, Vasculotropin, vascular endothelial growth factor A

Citations for Recombinant Human VEGF 189 (aa 27-215) Protein (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human VEGF 189 (aa 27-215) Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Ma et al. (2021), CD63-mediated cloaking of VEGF in small extracellular vesicles contributes to anti-VEGF therapy resistance Cell Reports, 36(7):109549.
PMID: 34407412
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Jahangiri et al. (2017), Cross-activating c-Met/?1 integrin complex drives metastasis and invasive resistance in cancer Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 28973887

Recombinant Human VEGF 189 (aa 27-215) Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human VEGF 189 (aa 27-215) Protein

Formulation, Preparation and Storage

Carrier Free

8147-VE

Carrier

8147-VE/CF

Carrier Free

Reconstitution Calculator

Background: VEGF

Citations for Recombinant Human VEGF 189 (aa 27-215) Protein (2)

Product Documents for Recombinant Human VEGF 189 (aa 27-215) Protein

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