Recombinant Human VEGF 162 Protein

Name: Recombinant Human VEGF 162 Protein
Brand: R&D Systems
SKU: 2347-VE
Price: 388 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 2347-VE

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	P15692
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human VEGF 162 Protein

Source	Mouse myeloma cell line, NS0-derived human VEGF protein Ala27-Arg188
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala27
Predicted Molecular Mass	18.8 kDa (monomer)
SDS-PAGE	15-20 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 1.0-5.0 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

2347-VE

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2347-VE/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

The human VEGF-A gene contains eight exons plus a nontraditional ninth exon in the 3’ UTR (1). The A gene gives rise to multiple splice variants of VEGF-A, ranging from 121 amino acids (aa) to 206 aa in length. All human splice variants contain exons 1-5 and some part of exon 8. Exons 6 and 7 are split into at least two parts, some of which may be included in any one molecule (1-5). The nontraditional 9^th exon is a substitute for exon 8. Incorporation of the 9^th exon results in a non-signaling form of VEGF (5). VEGF₁₆₂ is a VEGF isoform that induces endothelial cell proliferation in vitro and angiogenesis in vivo (5). It is synthesized as a 40-45 kDa disulfide-linked homodimer whose subunits are a product of exons 1-5, 6a, 6b and 8 (5). The three aa difference between VEGF₁₆₅ and VEGF₁₆₂is attributable to the fact that the 165 form only utilizes exon 7 while the 162 form only utilizes exon 6. Within exon 6, exon 6a contributes 24 aa while exon 6b contributes 17 aa (aa 116‑156 in the mature segment) (2). Exon 6a is not commonly found with exon 6b. In VEGF₁₆₂, exon 6b appears to act as a counterpart to the activity of exon 6a. That is, the product of exon 6a normally promotes VEGF binding to basement membrane while the product of exon 6b promotes VEGF dissociation and solubilization. In this case, VEGF₁₆₂ is notably soluble.

Item	Value
References	Ferrara, N. (2004) Endocrine Rev. 25:581. Lange, T. et al. (2003) J. Biol. Chem. 278:17164. Whittle, C. et al. (1999) Clin. Sci. 97:303. Jingjing, L. et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:752. Woolard, J. et al. (2004) Cancer Res. 64:7822.
Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names	MVCD1, VAS, VEGF, VEGF-A, VEGFA, VEGFMGC70609, VPF, VPFvascular endothelial growth factor, Vascular permeability factor, Vasculotropin, vascular endothelial growth factor A

Citations for Recombinant Human VEGF 162 Protein (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human VEGF 162 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Dikov MM et al. (2005), Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation. J. Immunol., 174(1):215-22.
PMID: 15611243

Recombinant Human VEGF 162 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human VEGF 162 Protein

Formulation, Preparation and Storage

Carrier Free

2347-VE

Carrier

2347-VE/CF

Carrier Free

Reconstitution Calculator

Background: VEGF

Citations for Recombinant Human VEGF 162 Protein (2)

Product Documents for Recombinant Human VEGF 162 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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