Recombinant Human VEGF 145 (aa 27-171) Protein

Name: Recombinant Human VEGF 145 (aa 27-171) Protein
Brand: R&D Systems
SKU: 7626-VE
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7626-VE

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Key Product Details

Source:	E. coli
Accession #:	NP_001191313
Structure / Form:	Disulfide-linked dimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human VEGF 145 (aa 27-171) Protein

Product Specifications for Recombinant Human VEGF 145 (aa 27-171) Protein

Source	E. coli-derived human VEGF protein Ala27-Arg171, with an N-terminal Met
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met-Ala27 & Pro28
Predicted Molecular Mass	17 kDa (monomer)
SDS-PAGE	20 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED₅₀ for this effect is 12-60 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

7626-VE

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7626-VE/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

Vascular endothelial growth factor (VEGF or VEGFA), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family of proteins that is characterized by the presence of eight conserved cysteine residues that form a cystine knot structure (4). Humans express multiple alternatively spliced isoforms of VEGF that are 111, 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4, 5, 6). Isoforms other than VEGF₁₁₁ and 121 contain basic heparin binding regions and are not freely diffusible (4, 5). VEGF₁₆₅ appears to be the most abundant and potent isoform, followed by VEGF₁₂₁ and VEGF₁₈₉ (3-5). VEGF₁₄₅ expression is relatively low, and is found mainly in female and male reproductive tissues, carcinomas of the female reproductive system, and other solid tumors (5, 7, 8). Expression of mouse VEGF₁₄₄, the rodent ortholog to human VEGF₁₄₅, is particularly enhanced by metabolic stress, such as that encountered during sustained exercise or starvation (8, 9). Human VEGF₁₄₅ shares 89% aa sequence identity with mouse and rat VEGF₁₄₄. VEGF₁₄₅ binds to the type I transmembrane receptor tyrosine kinase VEGF R2/KDR/Flk1 on endothelial cells. This receptor appears to be the primary mediator of VEGF angiogenic activity (3-5, 7). Unlike VEGF₁₆₅, VEGF₁₄₅ does not bind VEGF R1/Flt1 or VEGF R3/Flt-4 (4, 5, 7). VEGF₁₄₅ also binds the semaphorin receptor Neuropilin2 and promotes complex formation with VEGF R2, but unlike VEGF₁₆₅ and VEGF₁₂₁, it does not bind Neuropilin1 (10, 11). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4, 7). In adults, VEGF acts mainly during wound healing and the female reproductive cycle (3). Pathologically, isoforms that include VEGF₁₄₅ are involved in tumor angiogenesis and vascular leakage (5, 12, 13).

Item	Value
References	Leung, D.W. et al. (1989) Science 246:1306. Keck, P.J. et al. (1989) Science 246:1309. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853. Woolard, J. et al. (2009) Microcirculation 16:572. Mineur, P. et al. (2007) J. Cell Biol. 179:1261. Poltorak, Z. et al. (1997) J. Biol. Chem. 272:7151. Zhang, L. et al. (2002) Biochem. Biophys. Res. Commun. 292:860. Ding, Y.H. et al. (2004) Curr. Neurovasc. Res. 1:411. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049. Gluzman-Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040. Weis, S.M. and D.A. Cheresh (2005) Nature 437:497. Thurston, G. (2002) J. Anat. 200:575.
Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names	MVCD1, VAS, VEGF, VEGF-A, VEGFA, VEGFMGC70609, VPF, VPFvascular endothelial growth factor, Vascular permeability factor, Vasculotropin, vascular endothelial growth factor A

Citations for Recombinant Human VEGF 145 (aa 27-171) Protein (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human VEGF 145 (aa 27-171) Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lecce et al. (2014), Aging impairs VEGF-mediated, androgen-dependent regulation of angiogenesis. Mol Endocrinol, 28(9):1487-501.
PMID: 25058601

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Recombinant Human VEGF 145 (aa 27-171) Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human VEGF 145 (aa 27-171) Protein

Formulation, Preparation and Storage

Carrier Free

7626-VE

Carrier

7626-VE/CF

Carrier Free

Reconstitution Calculator

Background: VEGF

Citations for Recombinant Human VEGF 145 (aa 27-171) Protein (1)

Customer Reviews for Recombinant Human VEGF 145 (aa 27-171) Protein (1)

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