Recombinant Human VEGF 145 (aa 27-171) Protein

R&D Systems, part of Bio-Techne | Catalog # 7626-VE

R&D Systems, part of Bio-Techne
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Carrier Free
7626-VE-010/CF
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With Carrier
7626-VE-010
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human VEGF 145 (aa 27-171) Protein (7626-VE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession #

NP_001191313

Structure / Form

Disulfide-linked dimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VEGF Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human VEGF protein
Ala27-Arg171, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met-Ala27 & Pro28

Predicted Molecular Mass

17 kDa (monomer)

SDS-PAGE

20 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.
The ED50 for this effect is 12-60 ng/mL.

Reviewed Applications

Read 1 review rated 4 using 7626-VE in the following applications:

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7626-VE
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7626-VE/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular endothelial growth factor (VEGF or VEGFA), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family of proteins that is characterized by the presence of eight conserved cysteine residues that form a cystine knot structure (4). Humans express multiple alternatively spliced isoforms of VEGF that are 111, 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4, 5, 6). Isoforms other than VEGF111 and 121 contain basic heparin binding regions and are not freely diffusible (4, 5). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3-5). VEGF145 expression is relatively low, and is found mainly in female and male reproductive tissues, carcinomas of the female reproductive system, and other solid tumors (5, 7, 8). Expression of mouse VEGF144, the rodent ortholog to human VEGF145, is particularly enhanced by metabolic stress, such as that encountered during sustained exercise or starvation (8, 9). Human VEGF145 shares 89% aa sequence identity with mouse and rat VEGF144. VEGF145 binds to the type I transmembrane receptor tyrosine kinase VEGF R2/KDR/Flk1 on endothelial cells. This receptor appears to be the primary mediator of VEGF angiogenic activity (3-5, 7). Unlike VEGF165, VEGF145 does not bind VEGF R1/Flt1 or VEGF R3/Flt-4 (4, 5, 7). VEGF145 also binds the semaphorin receptor Neuropilin2 and promotes complex formation with VEGF R2, but unlike VEGF165 and VEGF121, it does not bind Neuropilin1 (10, 11). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4, 7). In adults, VEGF acts mainly during wound healing and the female reproductive cycle (3). Pathologically, isoforms that include VEGF145 are involved in tumor angiogenesis and vascular leakage (5, 12, 13).

References

  1. Leung, D.W. et al. (1989) Science 246:1306.
  2. Keck, P.J. et al. (1989) Science 246:1309.
  3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
  4. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
  5. Woolard, J. et al. (2009) Microcirculation 16:572.
  6. Mineur, P. et al. (2007) J. Cell Biol. 179:1261.
  7. Poltorak, Z. et al. (1997) J. Biol. Chem. 272:7151.
  8. Zhang, L. et al. (2002) Biochem. Biophys. Res. Commun. 292:860.
  9. Ding, Y.H. et al. (2004) Curr. Neurovasc. Res. 1:411.
  10. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.
  11. Gluzman-Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040.
  12. Weis, S.M. and D.A. Cheresh (2005) Nature 437:497.
  13. Thurston, G. (2002) J. Anat. 200:575.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

UniProt

NP_001191313

Additional VEGF Products

Product Documents for Recombinant Human VEGF 145 (aa 27-171) Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human VEGF 145 (aa 27-171) Protein

For research use only

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