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Recombinant Human TWEAK R/TNFRSF12 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1199-TW

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1199-TW-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TWEAK R/TNFRSF12 protein
Human TWEAK R
(Glu28-Trp79)
Accession # Q9NP84
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu28

Predicted Molecular Mass

32 kDa (monomer)

SDS-PAGE

35-45 kDa, reducing conditions

Activity

Measured by its ability to inhibit the TWEAK-dependent proliferation of HUVEC human umbilical vein endothelial cells.
The ED50 for this effect is 10-30 ng/mL in the presence of 15 ng/mL recombinant human TWEAK.

Formulation, Preparation and Storage

1199-TW
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TWEAK R/TNFRSF12

The gene for TNF-related weak inducer of apoptosis receptor (TWEAK R) was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Human TWEAK R cDNA encodes a 129 amino acid (aa) residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1 - 3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain conatins one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death, cell proliferation, and angiogenesis (2 - 6). In new born mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

References

  1. Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
  2. Feng, S. et al. (2000) Am J. Pathol. 156:1253.
  3. Wiley, S. et al. (2001) Immunity 15:837.
  4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
  5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
  6. Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.

Long Name

TNF-like and Weak Inducer of Apoptosis Receptor

Alternate Names

CD266, Fn14, TNFRSF12, TNFRSF12A, TWEAKR

Entrez Gene IDs

51330 (Human); 27279 (Mouse)

Gene Symbol

TNFRSF12A

UniProt

Additional TWEAK R/TNFRSF12 Products

Product Documents for Recombinant Human TWEAK R/TNFRSF12 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human TWEAK R/TNFRSF12 Fc Chimera Protein, CF

For research use only

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