Recombinant Human Tsukushi/TSK Protein, CF

Name: Recombinant Human Tsukushi/TSK Protein, CF
Brand: R&D Systems
SKU: 3940-TS

Catalog # 3940-TS | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	Q8WUA8
Applications:	Binding Activity

View Full Technical Details for Recombinant Human Tsukushi/TSK Protein, CF

Technical Data
Citations (1) / Reviews

Product Specifications for Recombinant Human Tsukushi/TSK Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Tsukushi/TSK protein Thr17-Leu353, with a C-terminal 6-His tag
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Thr17
Predicted Molecular Mass	40 kDa
SDS-PAGE	40 kDa
Activity	Measured by its binding ability in a functional ELISA. Immobilized rhBMP-4 at 1 µg/mL can bind rhTSK with an apparent K_D <15 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3940-TS

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl. Reconstitution Buffer Available: Size / Price Qty Loading... RB03:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Tsukushi/TSK

TSK, also known as Tsukushi, is an atypical member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins (1, 2). All contain leucine-rich repeats (LRR) flanked by conserved cysteines, but the C-terminus of TSK resembles the structure of nyctalopin and chondroadherin rather than class I - III SLRPs (1). The human TSK cDNA encodes a 16 amino acid (aa) signal sequence and a 337 aa secreted (presumably glycosylated) protein that contains nine LRR (2). Proteoglycan modification of TSK has not been shown. SLRP-like activity or expression of TSK has been identified in several contexts. TSK mRNA is upregulated by estrogen treatment in breast cancer cell lines, and so is proposed to be involved in hormonally regulated extracellular matrix remodeling (2). TSK is upregulated along with bone markers in Vitamin K2-treated osteosarcoma cell lines, mediated by the pregnane X receptor, PXR (3). TSK was also shown to contribute to vitamin K2-mediated enhancement of collagen accumulation. Other SLRPs have been shown to form a protective coat around collagen fibrils, shielding them from proteolysis (4). In rats, expression of TSK in liver, intestines and islets of Langerhans resembles the pattern of Glut2 (5). TSK transcription is upregulated by both estrogen and insulin in hepatocytes. In Xenopus and chick embryos, TSK is expressed in the middle primitive streak, where it binds and antagonizes BMP-4 and promotes dorsalization and formation of the neural crest and the primitive organizer (6, 7). Xenopus TSK also binds the extracellular region of delta-1, modulating Notch activity (7). Human TSK shows 87%, 86%, 86%, 85%, 52% and 50% aa identity with canine, mouse, rat, bovine, chick, and Xenopus TSK, respectively, with the highest identity in the first four LRR.

Item	Value
References	McEwan, P.A. et al. (2006) J. Struct. Biol. 155:294. Charpentier, A.H. et al. (2000) Cancer Res. 60:5977. Ichikawa, T. et al. 2006, J. Biol. Chem. 281:16927. Geng, Y. et al. (2006) Matrix Biol. 25:484. Coffy, S. et al. (2005) Biochem. J. 385:165. Ohta, K. et al. (2004) Dev. Cell 7:347. Kuriyama, S. et al. (2006) Development 133:75.
Long Name	Tsukushin Leucine-rich Repeat Containing Protein 54
Entrez Gene IDs	25987 (Human)
Alternate Names	E2IG4, E2IG4E2-induced gene 4 protein, LRRC54, Leucine-rich repeat-containing protein 54, TSK, TSKU, TSKtsukushi homolog, Tsukushi, leucine rich repeat containing 54, tsukushi small leucine rich proteoglycan homolog (Xenopus laevis), tsukushi, small leucine rich proteoglycan, tsukushin

Citations for Recombinant Human Tsukushi/TSK Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Tsukushi/TSK Protein, CF include:

Applications: ELISA Control

M Furuhashi et al. (2022-03-10), Plasma Tsukushi Concentration Is Associated with High Levels of Insulin and FGF21 and Low Level of Total Cholesterol in a General Population without Medication Metabolites, 2022-012(3).
PMID: 35323680

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