Product Specifications for Recombinant Human TSLPR His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human TSLPR protein Gln23-Lys231, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Gln23 inferred from enzymatic pyroglutamate treatment revealing Gly24
Predicted Molecular Mass
37-47 kDa, under reducing conditions.
Measured by its binding ability in a functional ELISA. When Recombinant Human TSLPR His-tag is immobilized at 1.00 μg/mL (100 μL/well), Recombinant Human TSLP (Catalog # 1398-TS) binds with an ED50 of 0.200-1.20 ng/mL.
Scientific Data Examples for Recombinant Human TSLPR His-tag Protein, CF
Recombinant Human TSLPR His-tag Protein Binding Activity.
When Recombinant Human TSLP R His-tag (Catalog # 10963-TR) is immobilized at 1.00 μg/mL (100 μL/well), Recombinant Human TSLP (1398-TS) binds with an ED50 of 0.200-1.20 ng/mL.
Recombinant Human TSLPR His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human TSLPR His-tag Protein (Catalog # 10963-TR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-47 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Thymic stromal lymphopoietin receptor (TSLPR), also known cytokine receptor-like module 2 (CRLM-2) and IL-XR, is a member of the type 1 cytokine receptor family. TSLPR has been identified most closely related to the common gamma chain ( gamma c) and, when complexed with interleukin 7 receptor alpha (IL-7R alpha ), forms a high affinity complex for the IL-7-like cytokine TSLP (1-4). The extracellular domain (ECD) of human TSLPR contains two fibronectin type III-like domains and a WSXWS‑like motif, which is necessary for proper protein folding (1-4). The cytoplasmic domain contains a membrane-proximal box 1 motif that is important for association with JAKs (2, 3). The ECD of Human TSLPR shares 34% amino acid sequence identity with the ECD of mouse TSLPR. An alternatively spliced mRNA variant encoding a soluble TSLPR has also been reported in mouse (5). In the signaling pathway of TSLP-TSLPR, which is similar to that of IL-7, TSLP activates the transcription factor signal transducer and activator of transcription 3 (STAT3), inducing the expression of common genes (4). TSLPR expression is ubiquitous in the immune and hematopoietic cells but is up-regulated in Th2-skewed cells (3, 4). Elevated expression of TSLP-TSLPR in bronchial mucosa has been associated with human asthma by acting as a susceptibility factor to generate Th2 allergic responses to antigens (4, 6). TSLP also is involved in Th2-mediated allergic skin inflammation by inducing Th2 cytokine secretion by T cells during the effector phase of allergic skin inflammation (4, 7). TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (DC) (4). TSLP activated human DCs are also involved in the homeostatic proliferation of naïve and memory T cells in the absence of foreign antigens (4).
Park L.S. et al. (2000) J. Exp. Med. 192:659.
Blagoev, B. et al. (2002) Gene. 284:161.
Ziegler, S.F. et al. (2013) Advances in Pharmacology. 66:129.
He, R. et al. (2010) Ann N Y Acad Sci. 1183:13.
Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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