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Recombinant Human TSLP Protein Best Seller

Bio-Techne includes R&D Systems | Catalog # 1398-TS

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Carrier Free
1398-TS-010/CF

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With Carrier
1398-TS-010

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human TSLP protein
Tyr29-Gln159, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

15 kDa

Activity

Measured in a cell proliferation assay using BaF3 mouse pro‑B cells co-transfected with human IL-7 R alpha and human TSLP R. Reche, P.A. et al. (2001) J. Immunol. 167:336.
The ED50 for this effect is 0.05-0.3 ng/mL.

Scientific Data Images for Recombinant Human TSLP Protein

Recombinant Human TSLP Protein Bioactivity

Recombinant Human TSLP Protein Bioactivity

Recombinant Human TSLP (Catalog # 1398-TS) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with human IL-7 R alpha and human TSLP R. The ED50 for this effect is 0.05-0.3 ng/mL.
Recombinant Human TSLP Protein SDS-PAGE

Recombinant Human TSLP Protein SDS-PAGE

1 μg/lane of Recombinant Human TSLP was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1398-TS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1398-TS/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSLP

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells (1 - 3). The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gammac.

References

  1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
  2. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  3. Pandey, A. et al. (2000) Nature Immunol. 1:59.
  4. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  5. Quentmeier, H. et al. (2001) Leukemia 15:1286.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Entrez Gene IDs

85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)

Gene Symbol

TSLP

UniProt

Additional TSLP Products

Product Documents for Recombinant Human TSLP Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human TSLP Protein

For research use only

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  • Recombinant Human TSLP Protein
    Name: Anonymous
    Application: ELISA standard
    Verified Customer | Posted 06/09/2020
    We used this protein in an in-house ELISA along with mAb (1398) and pAb (AF1398) to quantify TSLP in human serum and plasma. This combination could not detect TSLP samples but generated a good standard curve.
    Recombinant Human TSLP Protein 1398-TS
  • Recombinant Human TSLP Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/06/2017

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